第三篇ACS上只是一篇摘要集中的一个摘要,以下是摘要内容:
66. Light Scattering Labels of DNA: Resonance Enhancement
from Bound Dyes. David A. Knoll and Victor Bloomfield.
Department of Biochemistry, University of Minnesota,
St. Paul, MN 55108.
According to resonance enhancement (RE) theory (Stanton,
S. G., et al. (1981) J. Chem. Phys. 75 (12), 5615), one can
expect large increases in both the polarized and depolarized
components of the scattered light when the wavelength of the
incident light is near a strong absorption band in the scattering
molecule. We have been investigating the feasibility of using
resonance enhancement from bound chromophores as a means
of specifically labeling macromolecules. We have chosen the
dye proflavine (PF) since it has (1) strong, well-characterized
binding to DNA, and (2) a strong absorption band (A, =
454 nm) which is near the 488-nm line of our Ar' laser. Using
429 DNA (MW = 11.5 M) and one PF bound every 2.3 base
pair, we have obtained over a 50-fold enhancement in the
polarized sacttering. Thus far, we have not observed enhancement
of the depolarized component. Over a range of
DNA concentrations from 0.06 to 2 mg/mL, the amount of
enhancement decreased with decreasing PF/DNA ratio, as
expected. Extension of these studies to other dyes and
wavelengths will be presented. We anticipate that the RE
technique will be useful in studying the scattering of DNA
at low concentration.