6.乙酸-乙酸钠缓冲液(0.2M)
pH 0.2M NaAc/ml 0.2M HAC/ml pH 0.2M NaAc/ml 0.2M
HAC/ml
3.6 0.75 9.25 4.8 5.90 4.10
3.8 1.20 8.80 5.0 7.00 3.00
4.0 1.80 8.20 5.2 7.90 2.10
4.2 2.65 7.35 5.4 8.60 1.40
4.4 3.70 6.30 5.6 9.10 0.90
4.6 4.90 5.10 5.8 9.40 0.60
NaAc分子量=136.09 0.2M溶液为27.22g/L
7.磷酸盐缓冲液
(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2M)
pH 0.2M Na2HPO4/ml 0.2M NaH2PO4/ml pH 0.2M
Na2HPO4/ml 0.2M NaH2PO4/ml
5.8 8.0 92.0 7.0 61.0 39.0
5.9 10.0 90.0 7.1 67.0 33.0
6.0 12.3 87.7 7.2 72.0 28.0
6.1 15.0 85.0 7.3 77.0 23.0
6.2 18.5 81.5 7.4 81.0 19.0
6.3 22.5 77.5 7.5 84.0 16.0
6.4 26.5 73.5 7.6 87.0 13.0
6.5 31.5 68.5 7.7 89.5 10.5
6.6 37.5 62.5 7.8 91.5 8.5
6.7 43.5 56.5 7.9 93.0 7.0
6.8 49.5 51.0 8.0 94.7 5.3
6.9 55.0 45.0
Na2HPO4·2H2O分子量=178.05 0.2M溶液含35.61g/L
Na2HPO4·12H2O分子量=358.22 0.2M溶液含71.64g/L
NaH2PO4·H2O分子量=138.01 0.2M溶液含27.6g/L
NaH2PO4·2H2O分子量=156.03 0.2M溶液含31.21g/L
(2) 磷酸氢二钠-磷酸二氢钾缓冲液(1/15M)
pH 1/15M Na2HPO4/ml 1/15M KH2PO4/ml pH 1/15M
Na2HPO4/ml 1/15M KH2PO4/ml
4.92 0.10 9.90 7.17 7.00 3.00
5.29 0.50 9.50 7.38 8.00 2.00
5.91 1.00 9.00 7.73 9.00 1.00
6.24 2.00 8.00 8.04 9.50 0.50
6.47 3.00 7.00 8.34 9.75 0.25
6.64 4.00 6.00 8.67 9.90 0.10
6.81 5.00 5.00 9.18 10.00 0
6.98 6.00 4.00
Na2HPO4·2H2O分子量=178.05 1/15M溶液含35.61g/L
KH2PO4分子量=136.09 1/15M溶液含9.078g/L
8.磷酸二氢钾-氢氧化钠缓冲液(0.05M)
X ml 0.2M KH2PO4 +Y ml 0.2M NaOH 再加水稀释至20ml
pH(20℃) X/ml Y/ml pH(20℃) X/ml Y/ml
5.8 5 0.372 7.0 5 2.963
6.0 5 0.570 7.2 5 3.500
6.2 5 0.860 7.4 5 3.950
6.4 5 1.260 7.6 5 4.280
6.6 5 1.780 7.8 5 4.520
6.8 5 2.365 8.0 5 4.680
9.巴比妥钠-盐酸缓冲液(18℃)
pH 0.04M巴比妥钠/ml 0.2M盐酸/ml pH 0.04M巴比妥钠/ml 0.2M盐酸/ml
6.8 100 18.4 8.4 100 5.21
7.0 100 17.8 8.6 100 3.82
7.2 100 16.7 8.8 100 2.52
7.4 100 15.3 9.0 100 1.65
7.6 100 13.4 9.2 100 1.13
7.8 100 11.47 9.4 100 0.70
8.0 100 9.39 9.6 100 0.35
8.2 100 7.21 100
巴比妥钠分子量=206.18 0.04M溶液为8.25g/L
10.Tris-盐酸缓冲液
50ml 0.1M三羟甲基氨基甲烷(Tris)溶液于X ml 0.1M盐酸混匀后,加水稀释至100ml
pH X/ml pH X/ml
7.1 45.7 8.1 26.2
7.2 44.7 8.2 22.9
7.3 43.4 8.3 19.9
7.4 42.0 8.4 17.2
7.5 40.3 8.5 14.7
7.6 38.5 8.6 12.4
7.7 36.6 8.7 10.3
7.8 34.5 8.8 8.5
7.9 32.0 8.9 7.0
8.0 29.2
三羟甲基氨基甲烷(Tris)分子量=121.14 0.1M溶液为12.114g/L
Tris溶液可以从空气中吸收二氧化碳,使用时注意将瓶盖严