【Author】 GAO Wang,ZHANG Ji-peng(1.The Affiliated Fourth Hospital of China Medical University,Shenyang 110004,China;2.Dandong Yichuang Medicine Corporation,Dandong 118000,China)
【Abstract】 Objective To establish an HPLC method for the determination of baicalin in Xiaoyan tablet.MethodsDiamonsil C18 Column(150 mm×4.6 mm,5 μm)was used.The mobile phase was methanol-0.2 mol/L sodium dihydrogen phosphate buffer(40∶60,adjusting the pH value with phosphoric acid).The detection wavelength was 275 nm at a flow rate of l.0 mL/min.ResultsThere was good linearity of baicalin in 0.201 0~0.704 2 μg(r=0.999 9),and the average recovery was 98.1%,RSD was 1.24%(n=5).ConclusionThe method is reliable... 更多还原 【关键词】 消炎片； 黄芩苷； 高效液相色谱法； 【Key words】 Xiaoyan tablet； Baicalin； HPLC；
【Author】 WANG Yan-hou1,JIA Lei2(1.Zibo Institute for Drug Control of Shandong Province,Zibo 255086,China;2.Zibo Science and Technology Vocational College of Shandong Province,Zibo 255314,China)
【Abstract】 Objective To establish an HPLC method for determination of ofloxacin in ofloxacin otic solution.MethodsThe Dikma Diamonsil C18 stainless steel column(4.6 mm×150 mm,5 μm)was used,the flow rate was 1.0 mL/min with the acetonitrile-0.1% triethy solution(14∶86)(pH was adjusted to 3.0 with phosphoric acid)as mobile phase,the column temperature was 35 ℃,and the Waters 996 PDA detection set wavelength was at 294 nm.ResultsThere was good linearity of the calibration carve between 0.153 9～3.078 0 μg,r=0.... 更多还原 【关键词】 HPLC； 氧氟沙星滴耳液； 氧氟沙星； 【Key words】 HPLC； Ofloxacin otic solution； Ofloxacin；
【Abstract】 Objective To establish a RP-HPLC method for determination of ferulid acid in Dangguilonghui Tablets.Methods The chromatography was carried out on a Diamonsil ODS column(200 mm × 4.6 mm,5 μm)with methanol-1%(φ)phosphate acid solution(120∶280,v/v)as the mobile phase at a flow rate of 1.0 mL /min.The detection wavelength was set at 316 nm.Results The calibration curve was linear within the range of 0.313 6～6.271 mg/L(r=0.999 5),the average recoveries was 100.5%(RSD=1.0%).Conclusion This method is a... 更多还原 【关键词】 高效液相色谱法； 当归龙荟片； 阿魏酸； 【Key words】 HPLC； Dangguilonghui Tablets； Ferulid acid；
【Author】 WANG Zhi-gang1,HUANG Yan2,YANG Hong-wu3,et al(1.Liaoning Province Pharmacy Industry Academe,Shenyang 110015,China;2.Liaoning Talsy Medicine Co.,Ltd,Shenyang 110001,China;3.Liaoning Tianfeng Industrial Sock Co.,Ltd,Shenyang 110027,China;4.Shenyang Baicao Medicine Co.,Ltd,Shenyang 110003,China)
【Abstract】 Objective To establish a method for the determination of gallic acid in Jingtiansanqi capsules.Methods HPLC was used.The determination was performed on a Diamonsil C18(200 mm×4.6 mm,5 μm)column by UV detector at 271 nm with methanol-0.6% Phosphoric acid(12∶88) as the mobile phase.The flow rate was 1.0 mL/min with column temperature of 40 ℃.Results The calibration curve showed a good linearity within the range of 160.1～800.5 ng(r=0.999 9).The average recovery was 99.05% with RSD of 0.88%(n=6).Con... 更多还原 【关键词】 景天三七胶囊； 没食子酸； 高效液相色谱法； 【Key words】 Jingtiansanqi capsules； Gallic acid； HPLC；
【Author】 ZONG Dong-sheng1,SHEN Chen2,LI Dan3,et al(1.Shenyang Pharmaceutical University,Shenyang 110016,China;2.Institute for Drug Control of Liaoyang City,Liaoyang 111000,China;3.Anshan Ju Li Da Pharmacy Co.,Ltd,Anshan 114000,China)
【Abstract】 Objective RP-HPLC method was established to determine chlorogenic acid and caffeic acid in Herba taraxaci.Methods Diamonsil C18 column was used with a mixture of acetonitrile and sodium phosphate buffer(13∶87,v/v) as mobile phase.Results The linear ranges were 2.4～48.0 μg/mL(r=0.999 5),1.0～20.0 μg/mL(r=0.999 7) respectively.The recoveries were 99.7%(RSD=0.8%),100.8%(RSD=1.1%) respectively.Conclusion The assay method is simple,rapid and with good reproducibility and,it could provide the quality a... 更多还原 【关键词】 蒲公英； 反相高效液相色谱法； 绿原酸； 咖啡酸； 【Key words】 Herba taraxaci； RP-HPLC； Chlorogenic acid； Caffeic acid；
【Abstract】 Objective To establish a method for determination of ecariine in Haimaduobian pill.Methods HPLC column C18(200 mm×4.6 mm,5 μm);mobile phase:acetonitrile-water(30∶70);detection wavelength:270 nm.Results A linear relationship of ecariine was good(0.073 0～0.730 μg,r=0.999 9).The average recovery was 99.89% with RSD=0.52%(n=6).Conclusion The method is simple,accurate,and it can be used for the quality control of Haimaduobian pill. 更多还原 【关键词】 海马多鞭丸； 淫羊藿苷； HPLC法； 【Key words】 Haimaduobian pill； Ecariine； HPLC；
【Abstract】 Objective To develop a RP-HPLC method for the determination of stavudine in the plasma of dogs.Methods Samples were treated with phosphate buffer(pH 6.0),and then were extracted by ethylester.The extract was injected into the HPLC system with an analytical column of Diamonsil C18(150 mm×4.6 mm,5 μm,ID) with the column temperature at room temperature.The mobile phase consisted of a mixture of water-methanol(80∶20,v/v) with a flow rate of 1.0 mL/min.The stavudine was detected by UV absorption at 2... 更多还原 【关键词】 司他夫定； RP-HPLC； 血药浓度； 含量测定； 【Key words】 Stavudine； RP-HPLC； Plasma concentration； Determination of content
【Author】 ZHOU Zhi-ming,HE Li-yuan(1.Department of Pharmacy,The First Hospital of China Medical University,Shenyang 110001,China; 2.Shengjing Hospital of China Medical University,Shenyang 110004,China)
【Abstract】 Objective To establish a HPLC method for the determination of the dissolution of Tiopronin tablet.Methods The dissolution of Tiopronin tablet was determinated by HPLC.Diamonsil(r) C18 column was used with methanol and water (5∶95) as mobile phase whose pH value was mediated to 2.5 by phosphoric acid,flow rate was 1.0 mL/min,the detecting wavelength was at 205 nm.Results The linear range was from 10 μg/mL to 100 μg/mL,r=0.999 9,the average recovery was 100.4%,RSD=1.0%(n=9).Conclusion This method ... 更多还原 【关键词】 硫普罗宁； 释放度； 高效液相色谱法； 【Key words】 Tiopronin； Dissolution； HPLC；
【Abstract】 Objective To establish a method for the determination of puerarin in Xiaoke capsules.Methods HPLC was used.Column:DiamonsilC18（5 μm,4.6 mm×200 mm）,mobile phase:acetonitrile-water（15∶85）,flow rate:1.0 mL/min,wavelength:306 nm.Results The linear range was 180.7～421.7 μg/mL（r=0.999 6）,the mean recovery was 100.6% and the RSD was 1.9%.Conclusion The method is rapid,simple and accurate. 更多还原 【关键词】 高效液相色谱法； 消渴胶囊； 葛根素； 【Key words】 HPLC； Xiaoke capsules； Puerarin；
【Author】 QIU Feng1,2,XU Jing-hua2,ZHAO Li-mei1,HE Xiao-jing(1.Department of Pharmacy,Shengjing Hospital of China Medical University,Shenyang 110004,China;2.Shenyang Pharmaceutical Universityy,Shenyang 110016)
【Abstract】 Objective A rapid sensitive HPLC method was developed to determine baicalin in the rat plasma.Methods The rat plasma was pretreated by precipitating with methanol-acetonitrile.Diamonsil C18 column was used.The mobile phase consisted of methanol-0.2% phosphoric acid(60:40,v/v).The flow rate was 1.0 mL/min,and the UV detection was set at 280 nm.CBZ was used as internal standard.Results The linear calibration curves were obtained in the concentration range of 0.25～10.0 μg/mL(r=0.999 0).The within-d... 更多还原 【关键词】 黄芩苷； HPLC； 血浆； 【Key words】 Baicalin； HPLC； Plasma；
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