Phosphate buffered saline From Wikipedia, the free encyclopedia Jump to: navigation, search
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).
Applications PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used as a cellular cleaning solution. To ensure the prolonged dry-storage of immobilized-biomolecules like proteins, enzymatic proteins etc., PBS is used as biomolecule diluent since it can structure water around biomolecules immobilized to the solid surface. Such thin film of water prevents denaturing of biomolecules or conformational changes to them. Carbonate buffers may be used for the same purpose but with less effectiveness. PBS can be used as a reference spectra when measuring the protein adsorption in ellipsometry.
Additives can be used to add function. For example, PBS with EDTA is also used to disengage attached and clumped cells. Divalent metals such as zinc, however, cannot be added as this will cause precipitation. For these types of applications, Good's buffers are recommended.
Preparation A 10 liter stock of 10x PBS can be prepared by dissolving 800 g NaCl, 20 g KCl, 144 g Na2HPO4 and 24g KH2PO4 in 8 L of distilled water, and topping up to 10 L. The pH is ~6.8, but when diluted to 1x PBS it should change to 7.4.
On dilution, the resultant 1x PBS will have a final concentration of 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4
Product Description Name: Phosphate Buffered Saline Buffer Chemical description: When reconstituded with water, PBS1x final concentrations are 137mM Sodium Chloride, 10mM phosphate, 2.7mM Potassium Chloride, pH is 7.4
Cat.number: UP68723A, 10L inquire for other quantities
Form: White powder, pack qsp 10L Use: reconstitute PBS 1x with distilled water: 1 pack (9.88g), qsp 10L of d.water mix; the solution should be clear and without particulates rem: One also may use 1pack with 1L to prepare 10X PBS mother solution
Storage: Room temperature (.)
Applications: PBS is used in various laboratory technics, including immunodetection,biochemistry, purification, cell culture (ask for our Sterile PBS)… Phosphate buffer should not be used in assays where competition for phosphate groups, or complex formation with a metal ion is essential for the enzyme activation. Phosphate ions will inhibit carboxypeptidase,carboxylase, urease, muscle diaminase, formase and phosphoglucomutase.
For any question, please contact Uptima, or our local distributor. Hotline : +33(0)4 70 03 76 06; uptima@interchim.com ; http://www.interchim.com
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