【Author】 ZHANG Qi,XIANG Guang-Ya,LONG Na,WU Ji-Zhou(School of Pharmacy,Tongji Medical College of Huazhong University of Science & Technology,Wuhan 430030,China)
【Abstract】 AIM:To investigate enzymatic hydrolysis of N-（phenylacetyl） doxorubicin（DOXP） catalyzed by folate-conjugated penicillin-G acylase（PGA）.METHODS:The determination of prodrug DOXP and cleavage product doxorubicin was monitored by HPLC.A Diamonsil C18 column（250 mm×4.6 mm,5 μm） was used with 1 mL/min flow rate of 85% phosphoric acid in water（pH 2.4） with a gradient of acetonitrile created according to the following scheme:24% for 3 min and then constant at 80% for 12 min.RESULTS:PGA effec... 更多还原 【关键词】 叶酸； 青霉素G酰化酶； N-苯乙酰化阿霉素； 催化活性； 高效液相色谱法； 【Key words】 Folate； Penicillin-G acylase； N-(Phenylacetyl) doxorubicin； Catalytic activity； HPLC； 【基金】 国家自然科学基金资助项目(No.30300430)~~
【Author】 WU Yan 1,GUO Cheng-Ming 1,Zhang Sheng-Qiang 2 ( 1Tianjin Institute for Drug Control,Tianjin 300070; 2Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009,China)
【Abstract】 AIM:To establish an HPLC and evaporative light-scattering detection(ELSD) method for the related substance analysis of spectinomycin and content determination of spectinomycin fermented solution.METHOD: The Diamonsil C_ 18 column (250 mm×4.6 mm,5 μm) was used and the mobile phase consisted of 0.04 mol/L trifluoroacetic acid solution.The temperature of the drift tube was 50 ℃ and the gas pressure was 3.0× 105 Pa.RESULT: The chromatography system met the need of related substances analysis of spec... 更多还原 【关键词】 大观霉素； 高效液相色谱法； 蒸发光散射检测； 含量测定； 有关物质； 【Key words】 Spectinomycin； HPLC； Evaporative light-scattering detection； Content determination； Related substance；
【Abstract】 AIM To develop HPLC method for the determination of the main ingredients (Baicalin and Icariin) in Anti-allergy Capsules. METHODS The measurement conditions used as follows; For Baicalin, Diamosil C18 Column; mobile phase: 0. 05mol/L phosphate solution-Acetonitrile (70: 30) ; flow rate 1 ml/min; wavelength for measurement 277 nm;For Icariin, Spherisorb C18 column; mobile phase: Acetonitrile-water-acetate acid(22:78: 0. 1); flow rate 1 ml/min; wavelength for measurement 270 nm. The recovery rates... 更多还原 【关键词】 黄芩苷； 淫羊霍苷； 高效液相色谱法； 【Key words】 Baicalin； Icariin； HPLC；
【Abstract】 AIM The purpose is to develop a method for determining the contents of two different xanthone in Polygala fallax Hemsl. METHOD The method used Dikma Diamonsil C 18 column with MeOH-H 2O(65∶35) as mobile phase,0.9ml/min as flow-rate and 996-photodiode array detector at 245 nm. RESULTS The relative standard deviation of the repeatability were 0.96% and 2.6% respectively and the average recovery were 99.40% and 100.8% respectively. CONCLUSION The method was simple and had good liner relationship. 更多还原 【关键词】 黄花倒水莲； 酮； 反相高效液相色谱； 二级管阵列检测器； 【Key words】 Polygala fallax Hemsl.； Xanthone； RP-HPLC； DAD；
【Abstract】 AIM The purpose is to develop the analysis method of Icariin in Shenbao mixture; METHODS A HPLC method was adopted .The measurement condition used are as follows: Dikma Diamonsil ODS column; mobile phase: acetonitrile water acetic acid glacial(30∶70∶1); flow rate 1.0ml/min; wavelength for measure 270 nm; The conditions of extraction and determination were optimized. RESULT The relative standard deviation of the reproducibility was 2.11% and the average recovery rate was 97.79%. CONCLUSION The me... 更多还原 【关键词】 肾宝合剂； 淫羊藿苷； 高效液相色谱法； 【Key words】 Shenbao mixture； Icariine； HPLC；
【Author】 DUAN Wei(Shenyang Pharmaceutical University,Shenyang 110016,China)DUAN Wei,XIA Dong-ya,GUO Tao(Dept. of Pharmacy,The General Hospital of Shenyang Military Region,Shenyang 110016,China)
【Abstract】 OBJECTIVE:To establish an HPLC method for the determination of cefepime in human plasma.METHODS:The separation of sample was carried on a Diamonsil C18 column with the mobile phase consisted of 20 mmol·L-1 ammonium acetate(which was adjusted to pH=5.00 by acetic acid)-acetonitrile(90∶10) at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm;the sample size was 20 μL,and the column temperature was set at room temperature.RESULTS:The linear range of cefepime was 0.2～50.0 μg·mL-... 更多还原 【关键词】 头孢吡肟； 高效液相色谱法； 血药浓度；
【Author】 YE Li-ka,XIE Zhi-hong,WANG Ruo-lun,XU Xin,ZHANG Wei-ting(The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China)
【Abstract】 OBJECTIVE:To determine the plasma mycophenolic acid concentration by HPLC, and study the multidoses pharmacokinetics character of mycophenolic acid in Chinese kidney transplantation patients. METHODS: The samples were precipitated with acetonitrile before injection. Diamonsil C18 column was used. The mobile phase consisted of acetonitrile-10 mmol·L-1 KH2PO4 (5∶6) at a flow rate of 1.1 mL·min-1. The detection wavelength was 254 nm and the column temperature was 40 ℃. This method was used to deter... 更多还原 【关键词】 霉酚酸； 高效液相色谱法； 肾移植患者； 血药浓度； 多剂量； 药动学； 【Key words】 Mycophenolic acid； HPLC； Kidney transplantation patients； Plasma drug concentration； Multidoses； Pharmacokinetics； 【基金】 广州市属高校科技项目(61027);广东省医院药学研究基金资助项目(2008A018)
【Author】 ZHANG Ya-zhou,LUO Li-li,DONG Li-sha(Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China)CAI Shao-qing(School of Pharmaceutical Sciences, Beijing University, Beijing 100083,China)
【Abstract】 OBJECTIVE: To establishes an HPLC method for determination of the contents of Osthol and Berberine in Kangfuyan effervescent suppository. METHODS: HPLC was carried out on a column of Diamonsil (250 mm×4.6 mm,5 μm) with mobile phase consisted of acetonitrile-0.2% phosphate acid (52∶48,Lauryl sulfonic acid sodium 0.1 g was contained in every 100 mL) with flow rate at 1 mL·min-1.The detection wavelength was set at 322 nm and the column temperature was set at room temperature. RESULTS: The linear ra... 更多还原 【关键词】 抗妇炎泡腾栓； 反相高效液相色谱法； 蛇床子素； 盐酸小檗碱； 含量测定； 【Key words】 Kangfuyan effervescent suppository； RP-HPLC； Osthole； Berberine； Content determination； 【基金】 贵州省中药现代化科技创新项目(2006-5011)
【Author】 WANG Xin(Department of Pharmacy,The Second Affiliated Hospital of Hebei Medical University,Shijiazhuang 050000,China)JIANG Qin(Center of Drug Research and Development,North China Pharmaceutical Group Corporation,Shijiazhuang 050015,China)
【Abstract】 OBJECTIVE:To establish the quality standard for Ruheneixiao soft capsules.METHODS:Radix Paeoniae Rubra,Radix Angelicae Sinensis and Spica Prunellae were identified using TLC.The content of Paeoniflorin in Ruheneixiao Soft Capsules was determined with HPLC.HPLC analytical method was carried out using a C18 column and a mixture containing 13 volume of acetonitrile and 87 volume of water as the mobile phase.The detection wavelength was set at 230nm.RESULTS:Spots obtained from the test solutions had... 更多还原 【关键词】 乳核内消软胶囊； 薄层色谱法； 高效液相色谱法； 赤芍； 当归； 夏枯草； 芍药苷； 【Key words】 Ruheneixiao soft capsules； TLC； HPLC； Radix Paeoniae Rubra； Radix Angelicae Sinensis； Spica Prunellae； Paeoniflorin；
【Abstract】 Objective: To develop an HPLC method for the determination of baicalin and arctiin in Huxitong Oral Solution.Method: Analysis was carried out at room temperature on a platisil ODS column(4.6 mm × 250 mm,5 μm) with acetonitrile-0.02% phosphoric acid as the mobile phase,detecting at 208 nm·Result: The average recovery of baicalin was 99.0%,RSD 0.94% and the linear range was 10-100 mg·L-1,r = 0.999 5;The average recovery of arctiin was 100.5%,RSD 1.29% and the linear range was 5-50 mg·L-1,r = 0.999... 更多还原 【关键词】 呼吸通口服液； 黄芩苷； 牛蒡子苷； 高效液相色谱法； 【Key words】 Huxitong Oral Solution； baicalin； arctiin； HPLC； 【基金】 南京市医学科技发展项目(YKK08109)
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