CHANG Lili , ZHANG Chunhui , XIAO Chuanbin, LAN Zunhai （1. College of Animal Husbandry ＆ Veterinary Science, He＇nan Agricultural University, Zhengzhou 450002 ,China; 2. Department of Animal Medicine, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China ）
To establish an HPLC method for determination the ceftiofur content of the Ceftiofur Suspension Injection （CSI）, the samples were separated on Diamonsil C18, （4.6 min × 250 mm,5um） column with the mobile phase of acetonitrile--phosphoric acid aqueous solution （φH3PO4 =0. 1%,28 ： 72, v/v）, flow rate 1.0 mL/min and the detection wavelength set at 292 nm. Results showed that ceftiofur content of three CSI were 97.47% , 98.02%, and 97.25% of the labelled quantitative, respectively. The linear range of ceftiofur was 0. 0364 - 1. 456 ug （r = 0. 999 6）, the average recovery were 98.08%, 98.95%and 98.70%, and RSD were 1. 10%, 1. 12% and 0.94% （n=9）, respectively. In conclusion, the established RP-HPLC method of ceftiofur quantitation was accurate and reliable,and can be used to evaluate the quality of Ceftiofur Suspension Injection.
MA Bei-juan,LI Xiao-yu,YE Yi-ping （1.The People＇s Hospital of Jinhua,Jinhua 321000,Zhejiang,China;2.Institute of Materia Medica,Zhejiang Academy of Medical Sciences,Hangzhou 310013,Zhejiang,China）
Objective： To establish a method for determination of the content of Tenacissoside B in the roots of Marsdenia tenacissima.Methods：The analysis was carried out by RP-HPLC on Diamonsil C18（250mm×4.6mm,5 μm）,Methanol∶water（78∶22） as mobile phase;wave length was 217 nm;flow rate was 1.0mL/min;column temperature was 30℃.Results： The liner range of Tenacissoside B was 1.01～10.1μg.Regression equation was： y=432.41x-13.14,R2= 0.9998.The average recovery was 98.30% with RSD=1.78%（n=6）.Conclusion： The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis for the stems of Marsdenia tenacissima.
Objective To develop a RP-HPLC method for determination of urapidil in sustained-release urapidil capsules. Methods A reversed phase HPLC method was carried out on a Diamonsil C18 column with UV detection at 268 nm. The mobile phase was methanol-acetonitrile-water （2 % pH6.0 phosphate buffer, V： V： V = 55：20：45） with the flow rate of 1.0 mL· min^-1. The content of urapidil is calculated by external standardization method. Result A good linear relationship for urapidil was obtained within the range of 2-20 mg· L^-1 （r = 0. 999 8）. The average recovery was 99.7 %, RSD was 1.6 % （n = 5）. Conclusion The method is proved to be simple and specific, and not interfered by excipients. It is accurate for quality control in sustained-release urapidil capsule.
CHENG Hua, XIONG Bin, DING Bi-jun, ZHU Wei, HUANG Long,YU Long-jiang（Technical Research ＆ Development Center, China Tobacco Hubei Industrail Co. Ltd., Wuhan 430051, China;College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China）
OBJECTIVE ： To establish a RP - HPLC method for the determination of dehydrocavidine in Yanhuanglian injection.METHODS： The determination was performed on a Dikma Diamonsil C18 column（250 mm × 4.6 mm, 5 μm） .The mobile phase was acetonitrile- water（40 ： 60） supplemented with 3.4 g · L ^-1 KH2PO4 and 1.7 g · L^- 1 sodium dodecylsulphate （SDS） with a flow rate of 1 mL · min^ -1.The chromatogram was detected at 345 nm, and the column temperature was kept at 25 ℃. RESULTS： The calibration curve of dehydrocavidine was linear （r = 0.999 1） within a range of 0.142-2. 840μg.The average recovery of dehydrocavidine was 99.70% （RSD = 1.68%, n = 9）. CONCLUSION：The established method is accurate, reliable and reproducible,and it can be used for the quality control of Yanhuanglian injection.
我的社区
签到
