Low temp.: 1, chemical instability for some components at higher temp.; 2, Following a low temperature reaction; 3, Following exchange processes or conformational change.
High temp.: 1, Solubility for some components which have low solubility at low temp. 2, Broad peaks (higher temp. will narrow down peaks); 3, Following a high temperture reaction; 4, Following exchange processes or conformational change at high temp.
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