主题：【求助】咨询有关瓦里安Cary 50型紫外软件问题

Cary50好像只有4种校正方式吧：
1）不校正
2）100％T校正
3）100％T和0％T校正
4）0％T校正和已知反射率参比校正

1.None
Select this if you do not require Baseline Correction to occur.

Baseline Correction

Select this option to apply a baseline correction (Baseline) to the sample scan (S) that is collected from the instrument as transmission values.

This correction is thus:

(S)/(Baseline)

Zero/Baseline Correction

Select this option to apply a 100%T baseline correction (100%TBaseline) and a zero line correction (0%TBaseline) to the sample scan (S). This correction is performed with the raw transmission data and is thus:

((S)-(0%TBaseline))/(100%TBaseline-0%TBaseline)

With this option, the Cary will prompt you to perform a 100%TBaseline first, followed by a 0%TBaseline when you perform a baseline collection.

This baseline option corrects for any inherent variations in the electronic zero line of the instrument. You should use this baseline option if your samples have areas of very low transmission (high absorbance) as any variations in the instrument's zero line will effect your measurements if they you do not correct for these. You should refer to ASTM method E903 for further details.

Zero SRA correction Cary 100/300/400/500/500i/4000/5000/6000"no"Cary 50 

Select this option to apply a 100 %T baseline correction (100%TBaseline) and a zero line correction (0%TBaseline) to a sample (S) measured with an Absolute VW Specular Reflectance Accessory (SRA).

Note
The VN SRA and the single bounce relative SRA accessories should not use this correction. Select a Baseline/Zero correction instead.

This correction is performed with the raw transmission data and, due to the double bounce on the sample, is thus:

Sqrt(((S)-(0%TBaseline))/(100%TBaseline-0%TBaseline))

With this option, the Cary will prompt you to perform a 100%TBaseline first, followed by a 0%TBaseline when you perform a baseline collection. You should refer to ASTM method E903 for further details.

Zero *Std. Ref. Correction

Select this option to apply a 100 %T baseline (100%TBaseline) correction and a zero line correction (0%TBaseline) to a sample (S) measured with a reflectance accessory which is not absolute (e.g. a Diffuse Reflectance Accessory or the Variable Angle Specular Reflectance accessory). The Cary then multiplies the result by the appropriate value in the 'Baseline Stdref' file which are the transmission values associated with the Standard Reference material you used to establish the baseline. Refer to the help for the Diffuse Relectance accessories for further details about Standard Reference materials.

This correction is performed with the raw transmission data and is thus:

((S)-(0%TBaseline))/(100%TBaseline-0%TBaseline)) x (Baseline Stdref)

With this option, the Cary will prompt you to perform a 100%TBaseline first, followed by a 0%TBaseline when you perform a baseline collection.

Note
If you select this option the Baseline button in the main window will not be enabled until you have retrieved a Std Ref file. Click this button to browse for and select a Std Ref file. This file must be in the ASCII *.CSV file format.

可以使用Cary Help，上面就是。

我感觉：
1.Baseline Correction
100%T基线校准
2.Zero/Baseline Correction
0%T和100%（以空气为参比）两种情况下校准
3.Zero SRA correction
Cary 50没有此项功能，因为Cary 50是单波长的仪器。
4.Zero *Std. Ref. Correction
0%T基线校准和100%T标准参比溶液校准。

Cary Help上面还有这些的别的说明，目录如下：
Software Application
  Scan
    Software User Interface
      Buttons
        Setup
          Baseline

Cary Help 里面有详细的公式，你自己可以参考。

你列的是cary5000和6000i的基线吧？
应该是做漫反的时候用3，其余的没怎么用过。