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Determination of poly(ethylene glycol) 300 in long chain free fatty acid mixtures by reversed-phase high-performance liquid chromatography




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Alister D. Muir*, Martin J. Reaney and Andrew J. Aubin

Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Sask. S7N 0X2, Canada


Received 15 December 1997; revised 27 March 1998; accepted 31 March 1998. Available online 25 June 1998.

Abstract
A rapid sensitive method has been developed for the detection and quantitation of poly(ethylene glycol) 300 (PEG 300) in long-chain free fatty acid mixtures that requires minimal sample preparation. The PEG 300 was separated from the free fatty acids by RP-HPLC using a water–tetrahydrofuran gradient. PEG and the free fatty acids were detected using evaporative light scattering detection. The minimum detectable level of PEG in a free fatty acid mixture was 0.0125%.

Author Keywords: Poly(ethylene glycol); Fatty acids