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原文由 wh2008(wh2008) 发表:
最近在用waters的hilic做糖苷类的，奇怪的问题是：
1> 十种氨基糖苷类的物质保留时间是一样的
2> 如果使用梯度的话，一种物质会有两个保留时间
如果哪位大侠在做氨基糖苷类，能否给个方法参考下？
| Column | Cogent UDC Cholesterol™, 4µm, 100A |
| Catalog No. | 69069-7.5P |
| Dimensions | 4.6 x 75 mm |
| Mobile Phase | 60:40 acetonitrile/DI water + 0.5% formic acid |
| Temperature | Ambient |
| pH | 2.90 |
| Flow rate | 0.5 mL/minute |
| Injection Volume | 1 µL |
| Samples | Uracil (m/z 113), Tobramycin (m/z 468), 1 mg/L of each dissolved in DI water |
| Detection | Mass Spec: Atmospheric Pressure Chemical Ionization in positive mode: APCI+ Single Ion Monitoring |
原文由 babyfast(babyfast) 发表:
我也打算拿这根柱子做下草甘膦和百草枯大家多交流，也发几个文章评职称呵呵
| Column | Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. | 70000-15P-2 |
| Dimensions | 2.1 x 150 mm |
| Solvents | A: DI water + 5 mM ammonium acetate B: 90% acetonitrile/10% DI water/10 mM ammonium acetate |
| Gradient | 0.0 min ?80.0%B 1 .0 min 80%B 1.1 min to 5%B 5.0 min 5%B 6.0 min to 80%B |
| Post time | 5 min |
| Flow rate | 0.5 mL/min. |
| Sample Prep | Glyphosate: 168.0067m/z (M - H)- Figure A: injection #1, RT = 3.365 min Figure B: injection #5, RT = 3.366 min Sample stock solution was purchased from Sigma (1000 mcg/mL). Sample for analysis was made by diluting the stock 1:100 in 30:70 solution A and B. |
| Detection | ESI ?neg - Agilent 6210 MSD TOF mass spectrometer. |
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