原文由 qingqingcao(qingqingcao) 发表:
Internal standard solution— Dissolve a suitable quantity of tributyrin, accurately weighed,
in chloroform, and dilute quantitatively with chloroform to obtain a solution having a
concentration of about 0.2 mg per mL.
内标溶液——用氯仿溶解适量三丁酸甘油酯(tributyrin),准确称量,使得内标溶液浓度为0.2mg/ml
原文由 qingqingcao(qingqingcao) 发表:
Standard solution— Transfer about 15 mg of glycerin and about 50 mg of tributyrin, both
accurately weighed, to a glass-stoppered, 25-mL conical flask; add 3 mL of Propionating
reagent; and heat at 75 for 30 minutes. Volatilize the reagents with the aid of a stream of
nitrogen at room temperature, add about 12 mL of chloroform, and mix. Dilute about 1 mL
of this mixture with chloroform to about 20 mL, and mix.
标准溶液——准确称取15mg甘油和约50mg三丁酸甘油酯到25ml容量瓶中,加入3ml丙酸试剂,75°加热30分钟,室温氮吹使之挥发干净,加入12ml氯仿,混匀。吸取1ml混合物到20ml容量瓶,氯仿定容,混匀。