目的:建立抗菌中药对临床常见病原性细菌抑菌作用的检测方法。方法:选择6种常用抗菌中药（黄连、苦参、连翘、大黄、生地、知母)，分别采用水提法制备至质量浓度1mg/L。应用液体稀释法测定上述中药对金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌的最低抑菌浓度(MIC)。结果:建立了中药抑菌作用的检测方法。黄连、大黄与连翘抑制细菌的作用较强，MIC值均≤0.05mg/L，其中黄连对金黄色葡萄球菌的抑菌作用最强（MIC值为0.00625mg/L）。结论:应用本方法可以进行中药抑菌作用的检测。抗菌中药对所试验菌种表现不同的抑菌作用。 【关键词】中草药；微生物敏感性试验；抗菌药；细菌 Study on Antibacteral Effects of Six Chinese Herbs Against Pathogenic Bacteria XUE Jianjiang1,QIAO Haixia2,LIU Jinjun2,et al 1.Clinicial Lab,The First Affilited Hospitial,Hebei North University,Zhangjiakou,075000,Hebei,China 2.Department of Microbiology,Hebei North University,Zhangjiakou,075000,Hebei,China 【ABSTRACT】Objective:To establish a method to detect the antibacterialactivity of traditional chinese medicine against pathegenicbacteria.Methods:Six varieties of traditional chinese medicine(Coptisetc)were selected and extracted with pure water to make concentratedwith 1mg/L.Three different common pathogenic bacteria were used todetermine the minimal inhibitory concentration(MIC)in vitro with liquiddilution method to the selected medicine.Results:The methods wereestablished to detect the antibacterial activity of traditional chinesemedicine.Three herbs had inhibition effects on the strains oftest,among which Coptis Chinesis had the strongest effect and MIC was0.00625g/mL to Staphylococcus aureus.Conclusion:The methods can serveas a reference for the antibacterial activity of traditional chinesemedicine.The degree of inhibition effect for different traditionalchinese medicine on different bacteria were different. 【KEY WORDS】Chinese Herb,Microbial Sensitivity Tests,AntiBacterial Agents,Bacteria 中药几千年来为中国人民的健康做出了重大贡献，在现代预防和控制细菌性感染疾病中也发挥了积极作用。应用中药进行抗感染治疗具有副作用小，药物来源广泛，价格低廉等优势［1，2］。在清热解毒抗菌类中药中开发抗菌药物具有良好的应用前景。长期以来，对于抗菌中药作用机理的较少认识限制了其在临床中的应用。目前，尚无统一规范的中药抗菌作用评价方法。为此，本研究拟应用抗生素最小抑菌浓度检测方法，检测6种常用抗菌中药（大黄、苦参、连翘、黄连、生地、知母）对三种常见病原性细菌（金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌）的抑菌作用，建立一种可以规范的中药抗菌作用检测方法。
我的社区
签到
赛默飞实验室产品焕新计划有奖调研！
【七月征文】不一YOUNG实验“猿”
报名开启：ICS2024第十三届光谱网络会议！
推荐收藏！盘点中药材及饮片检测解决方案
