主题：【求助】（取消求助），谢谢各位帮忙！文献一篇，谢谢【Current Drug Delivery】【Gilmore, Ian R】【2006】

原文由 abcr 发表:
题目：Delivery Strategies for siRNA-mediated Gene Silencing
期刊：Current Drug Delivery
作者：Gilmore, Ian R.; Fox, Stephen P.; Hollins, Andrew J.;
      Akhtar, Saghir
年，卷，页码：2006，3 147-155
全文链接：http://www.ingentaconnect.com/content/ben/cdd/2006/00000003/00000002/art00003
谢谢大侠相助！

只能下来ABSTRACT

RNA interference (RNAi) represents a promising new gene silencing technology for functional genomics and a potential therapeutic strategy for a variety of genetic diseases. RNAi involves the targeted post-transcriptional degradation of messenger RNA thereby inhibiting the synthesis of the desired protein. This effectively leads to silencing of gene expression. The effectors of this process are short interfering RNA (siRNA) duplexes (~21-23nt) that are key intermediaries in the specific degradation of target mRNA following incorporation into the RNA-induced silencing complex (RISC) in the cytosol. However, due to the large molecular weight and negative charge of siRNA duplexes the effective cellular up-take and intracellular delivery appear to represent a major challenge for the widespread use of RNAi in vivo. This review summarises some of the main delivery strategies that have been attempted for the transfection of siRNA to cells in vitro and in vivo.