求一篇文献:"Advances in the high-performance liquid chromatographic determination of phenylthiocarbamyl amino acids ".是"Journal of Chromatography A ,Volume 661, Issues 1-2, 11 February 1994, Pages 43-50"上的.作者"I. Molnár-Perl ".
Summary An HPLC system has been developed in which 23 phenylthiocarbamyl (PTC) amino acids are distinctly separated. Applying this system, the preceding steps in HPLC amino acid analysis i.e. gas-phase hydrolysis of the protein/peptide sample and the pre-column derivatization, were evaluated and optimized. PTC amino acids in HPLC buffer solution are stable for at least 16 hours if stored at 5°–8°C. The sensitivity of the method is in the low pmol range. Key Words Column liquid chromatography - Phenylthiocarbamyl amino acids - Pre-column derivatization - Gas-phase protein hydrolysis
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