原文由 a260 发表:
http://www.columbia.edu/~scb2001/tools/phosphate/phosphate.html
Phosphate Buffer Calculation A Javascript that calculates the amount of monosodium
phosphate and disodium phosphate necessary to
achieve a buffer at a given pH and strength.
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pH Buffer Strength mM
Monosodium phosphate, monohydrate grams/liter
Disodium phosphate, heptahydrate grams/liter
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How to use
Type the desired pH into the first cell, and type the intended buffer strength (in millimoles per liter) in the second cell. Press the calculate button, and the approximate percentages of monosodium phosphate, monohydrate and disodium phosphate, heptahydrate will be displayed. For example, a pH of about 7.4 with a buffer strength of 50 mM is obtained using 0.1558% monosodium phosphate monohydrate and 1.0374% disodium phosphate, heptahydrate.
How the calculation works
a260
![](https://simg.instrument.com.cn/bbs/images/brow/em17.gif)
, 咋找得这么准确啊!再找个中文的呀