近日我在用比色法做酪氨酸酶的抑制剂研究 具体方法如下： test reaction mixtures were prepared by adding 40 U of mushroom tyrosinase to 20 μl of plant extract, and then adding 40 μl of 1.5 mM L-tyrosine and 220 μl of 0.1 M sodium phosphate buffer (pH 6.5). The resulting mixture (300 μl) was incubated for 20 min at 30°C and absorption at 475 nm was measured. The same mixture, but without plant extract, was used as a control. The percent inhibition of tyrosinase activity was calculated as follows: %inhibition={[(A-B)-(C-D)/(A-B)}*100 A:OD at 475nm without test substance but with mushroom tyrosinase B:OD at 475nm without test substance and mushroom tyrosinase C:OD at 475nm with test substance and mushroom tyrosinase D:OD at 475nm with test substance but without mushroom tyrosinase
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