主题：【有奖征集】现代生命科学相关实验技术！

Supplements


The following supplementary materials are available to accompany Modern Genetic Analysis.

Solutions Manual

William Fixsen, Harvard University, 0-7167-3282-3.The Solutions Manual contains worked-out answers to all the problems in the textbook.

Modern Genetic Analysis Web Site

W. H. Freeman with Sumanas, Inc. with contributions from William Sofer, The State University of New Jersey at Rutgers. This multimedia learning tool complements and enriches the textbook. All the features of the CD-ROM function within the context of the book's coverage. Practice tools such as interactive quizzes in every chapter help students review for exams. The Modern Genetic Analysis Web site, at www.whfreeman.com/genetics, will be updated regularly.

Modern Genetic Analysis 1.0 CD-ROM. (hybrid format for Windows and Macintosh)

Packaged with every copy of the textbook, the Modern Genetic Analysis 1.0 CD-ROM is the same as the Web site with these two additions: (1) original animations on topics such as transcription, DNA replication, and complementation bring the textbook figures to life; and (2) for instructors, the text's entire illustration program and the original animations are available in a Presentation Manager, which allows the preparation of a series of illustrations, animations, and videos for lecture. Source files for the illustrations are provided so that images can be exported into presentation software programs.

Instructor's Resource Manual and Test Bank

Sally Allen, University of Michigan, and Ewen Harrison. Printed: 0-7167-3422-2, Windows: 0-7167-3421-4, Macintosh: 0-7167-3420-6

The Instructor's Resource Manual contains over 700 test questions in multiple-choice, true-false, and matching formats. It also contains complete sample exams and teaching hints. Electronic versions of the test questions let professors edit and rearrange questions and add their own.

Transparency Set: 0-7167-3423-0

A full-color overhead transparency set of 130 key illustrations from the textbook is available free of charge to qualified adopters. The overhead transparency set also includes 100 transparency masters of text illustrations and tables.



© 1999 by W. H. Freeman and Company. All rights reserved.

蛋白质纯化
　蛋白质分离纯化是用生物工程下游技术从混合物之当中分离纯化出所需要得目的蛋白质的方法。

　　是当代生物产业当中的核心技术。该技术难度、成本均高；例如一个生物药品的成本75%都花在下游蛋白质分离纯化当中。常用技术有：

　　１、沉淀，

　　２、电泳：蛋白质在高于或低于其等电点的溶液中是带电的，在电场中能向电场的正极或负极移动。根据支撑物不同，有薄膜电泳、凝胶电泳等。

　　３、透析：利用透析袋把大分子蛋白质与小分子化合物分开的方法。

　　４、层析：

　　a.离子交换层析，利用蛋白质的两性游离性质，在某一特定ＰＨ时，各蛋白质的电荷量及性质不同，故可以通过离子交换层析得以分离。如阴离子交换层析，含负电量小的蛋白质首先被洗脱下来。　

　　b.分子筛，又称凝胶过滤。小分子蛋白质进入孔内，滞留时间长，大分子蛋白质不能时入孔内而径直流出。

　　５、超速离心：既可以用来分离纯化蛋白质也可以用作测定蛋白质的分子量。不同蛋白质其密度与形态各不相同而分开。

我先支持一下新版主的说！呵呵～

支持新版主，同时也来向各位有经验的版油学习．

这个网址，可以看到一些东西：http://www.lmbe.seu.edu.cn/biology/bess/biology/default.htm

我最想知道我们的杂交水稻算不算是转基因食品。谁能解释一下？

国家推行众多的条例，再三强调，以人为本
以人的生命安全放在第一，
多了解点生命科学的内容很不错，
建议版主尽快整理，向广大版友展现。

以下网址为最新消息,供参考:
科学时报：写在人类基因组南方中心成立10周年之际:
http://www.sciencenet.cn/htmlnews/2008/11/213385.html
乐观科技功臣竟是无肾人:
http://news.sina.com.cn/s/2008-11-19/074814752189s.shtml

平时经常听到生命科学这个词，具体不太了解，希望斑竹多多介绍。

支持版主！
随着社会向前发展，高科学技术发展，生物环境（大气，辐射等因素）受到巨大挑战。是否发生物种（基因）变化，适者生存？