Figure 2. Multipipmaker (Schwartz et al. 2003) analyses of legumes and Arabidopsis (A, using Nicotiana as reference genome) and legumes (B, using Lotus as reference genome). Arrows indicate loss of rpl22 (1) and rps16 (2).
Repeat structure: - 67-191 direct & inverted repeats (≥ 30 bp, 90% homology) – mostly within intergenic spacer regions - Medicago has 191 repeats; Lotus has 67 repeats - Longest repeats are 274 (Lotus) & 287 (soybean) Both long repeats are in ycf2, 4 copies in the IR These conserved repeats are in the same location in all legumes, Solanaceae members or Arabidopsis – suggesting a functional role. Plant Molecular Biology (2005) 59: 309-322
Figure 4. Sequence alignment of IR loss region between psbA and ndhF for Medicago, Pisum, and Vicia. Shaded regions show genes and repeat elements. The presence the psbA & rbcL repeats in legumes that have lost one copy of the IR indicate that this loss has only occurred once during their evolutionary history. PDF 文
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