原文由 7336167 发表: for preparation of blood plates,add5-10%sterile, defibrinated blood to the sterile medium that has been cooled to45-50度
Because there is no space between the word “10%” and “sterile”, I suspected that some words might be missing especially when I found the following from https://www.dotscientific.com/pdf/tryptic_soy_blood_agar_base_2/trypticsoybloodagarbase2_7654.pdf :
Directions 1. Suspend 40 g of the medium in one liter of purified water. 2. Heat with frequent agitation and boil for one minute to completely dissolve the medium. 3. Autoclave at 121°C for 15 minutes. 4. Prepare 5 - 10% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45 - 50°C.
However, I just found that the original is correct from http://www.bd.com/ds/technicalCenter/inserts/Brucella_Media.pdf except a redundant comma.
我的社区
签到
赛默飞实验室产品焕新计划有奖调研！
【七月征文】不一YOUNG实验“猿”
报名开启：ICS2024第十三届光谱网络会议！
推荐收藏！盘点中药材及饮片检测解决方案
