主题：【求助】毒杀芬测定峰面积的计算问题

求助大虾们，谢谢了

用加标法确定哪个峰是毒杀芬,不可能这么多峰都是,最多几个异构体而以.

原文由 am1087 发表:
用加标法确定哪个峰是毒杀芬,不可能这么多峰都是,最多几个异构体而以.

纯标样出现的就是一堆峰，国标方法上的图也是这样，说明这一堆峰应该都是，但就是没有具体说明如何定义峰面积。

大家帮帮忙呀

你这个根本就没的算，走的乱七八糟的，如果标准物质是像你出的那样似的毛刺峰的话，那做标准的人应该先下岗。或者你换检测器，用质谱打一下。

原文由 tmlh 发表:
你这个根本就没的算，走的乱七八糟的，如果标准物质是像你出的那样似的毛刺峰的话，那做标准的人应该先下岗。或者你换检测器，用质谱打一下。

6楼的先看好再说，我看过国标，也进过标样，的确是这样的，我的想法是在这个时间段用面积归一法试试，单纯用峰面积外标法的话，是没法计算的。

如果一堆峰都是毒杀芬（没做过此农药），一般工作站或积分仪上面峰面积加和area sum功能，在第一个峰前area sum on,在最后一个峰area sum off,就可以把这一堆峰加在一体按总面积计算。

前一阵刚好也碰到过,英文名Toxaphene,实际是有200多个组分的混合物. 刚开始也是给吓了一跳.考虑了很久, 最后也就采用面积积分计算.

Toxaphene is a mixture of approximately 200 organic compounds, formed by the chlorination of camphene (2,2-dimethyl-3-methylenebicyclo[2,2,1]heptane, C10H16) to an overall chlorine content of 67-69 % by weight. The bulk of the compounds (mostly chlorobornanes, chlorocamphenes, and other bicyclic chloroorganic compounds) found in Toxaphene have chemical formulas ranging from C10H11Cl5 to C10H6Cl12, with a mean formula of C10H10Cl8. The formula weights of these compounds range from 308 to 551 grams/mole; the theoretical mean formula has a value of 414 grams/mole. Toxaphene is usually seen as a yellow to amber waxy solid. It is volatile enough to be transported for long distances through the atmosphere.

引自http://en.wikipedia.org/wiki/Toxaphene

原文由 jimzhu 发表:
如果一堆峰都是毒杀芬（没做过此农药），一般工作站或积分仪上面峰面积加和area sum功能，在第一个峰前area sum on,在最后一个峰area sum off,就可以把这一堆峰加在一体按总面积计算。

我试试，谢谢啦！

原文由 出来混迟早要还的(wintersun) 发表:
前一阵刚好也碰到过,英文名Toxaphene,实际是有200多个组分的混合物. 刚开始也是给吓了一跳.考虑了很久, 最后也就采用面积积分计算.

Toxaphene is a mixture of approximately 200 organic compounds, formed by the chlorination of camphene (2,2-dimethyl-3-methylenebicyclo[2,2,1]heptane, C10H16) to an overall chlorine content of 67-69 % by weight. The bulk of the compounds (mostly chlorobornanes, chlorocamphenes, and other bicyclic chloroorganic compounds) found in Toxaphene have chemical formulas ranging from C10H11Cl5 to C10H6Cl12, with a mean formula of C10H10Cl8. The formula weights of these compounds range from 308 to 551 grams/mole; the theoretical mean formula has a value of 414 grams/mole. Toxaphene is usually seen as a yellow to amber waxy solid. It is volatile enough to be transported for long distances through the atmosphere.

引自http://en.wikipedia.org/wiki/Toxaphene

标准上说毒杀芬是氯化莰烯，色谱上出的是驼峰，定性是根据保留时间窗口里的指纹特征峰，定量可以按照总峰面积算，也可以按其中的4-6个主峰来定量。感觉上如果样品净化效果不理想，以总峰面积计算可能会偏大，所以用4-6个特征主峰可能会好一点，具体的可以参考EPA 8081A  7.5.11至7.6.1.3.5