双向电泳常用溶液配方
urea | 8M | 0.48g |
CHAPS | 4% | 40mg |
DTT | 50~65mM | 7~9.8mg |
or TBP | 2mM | 10µl |
40% Bio-Lyte | 0.2%(w/v) | 5µl |
1%溴酚蓝 | 0.001% | 1µl |
MilliQ水 | 650µl |
urea | 7M | 0.42g |
thiurea | 2M | 0.152g |
CHAPS | 4% | 40mg |
DTT | 50~65mM | 7~9.8mg |
or TBP | 2mM | 10µl |
40% Bio-Lyte | 0.2%(w/v) | 5µl |
1%溴酚蓝 | 0.001% | 1µl |
MilliQ水 | 650µl |
Final concentration Amount
Bromophenol blue | 1% | 10mg |
Tris-base | 50mM | 6mg |
MilliQ H2O |
| to 1ml |
|
|
|
Final concentration | Amount | |
1.5M Tris-cl,pH8.8 Urea (Fw 60.06) Glycerol(87% v/v) SDS(Fw 288.38) 1%Bromophenol blue solution | 50mM 6M 30%(v/v) 2%(w/v) 0.002%(w/v) | 6.7ml 72.07g 69ml 4.0g 400μl To 200ml |
Final concentration | Amount | |
Acrylamide | 30% | 150g |
N,N’-methylenebisacrylamide or PDA | 0.8% | 4.0g 5.0g |
MilliQ H2O | To 500ml |
Final concentration | Amount | |
Tris-base(Fw 121.1) MilliQ H2O HCl(Fw 36.46) | 1.5M | 181.5g 750ml Adjust to pH8.8 |
MilliQ H2O | To 1000ml |
Final concentration | Amount | |
Ammonium persulphate(APS) MilliQ H2O | 10% | 1g To 10ml |
Final concentration | Amount | |
Tris base Glycine SDS MilliQ H2O | 25 mM 192mM 0.1%(w/v) | 15.1g 7.55g 72.1g 36.05g 5.0g 2.5g To 5000ml To 2500ml |
Final concentration | Amount | |
TGS electophoresis buffer Agrose (NA or M) 1% Bromophenol blue | --- 0.5% 0.002%(w/v) | 50ml 0.25g 100μl |