普遍地IgG的等电点为8.0，在pH＝8的情况下不结合基团。 但是如果考虑到IgG分为4个亚型，每个亚型的等电点又各有不同，分布范围很广，至少从6到9都有 IgG的等电点为pI 8.0，IgM，IgA的等电点为pI 7.0，7.4，在pH 8.0时IgG带正电荷 I found the heavy chain sequence of RAT IgG2 and the light chain sequence of MOUSE kappa chain and the predicted PI is 7.2. This is how you calculate the PI of a protein. If I put in MOUSE lambda light chain sequence, the predicted PI is 8.8.
I did not dig deeply but I did not find the light chain sequence of rat light chain. Mouse and rat light chain should have very similar sequence.
普遍地IgG的等电点为8.0，在pH＝8的情况下不结合基团。 但是如果考虑到IgG分为4个亚型，每个亚型的等电点又各有不同，分布范围很广，至少从6到9都有 IgG的等电点为pI 8.0，IgM，IgA的等电点为pI 7.0，7.4，在pH 8.0时IgG带正电荷 I found the heavy chain sequence of RAT IgG2 and the light chain sequence of MOUSE kappa chain and the predicted PI is 7.2. This is how you calculate the PI of a protein. If I put in MOUSE lambda light chain sequence, the predicted PI is 8.8.
I did not dig deeply but I did not find the light chain sequence of rat light chain. Mouse and rat light chain should have very similar sequence.
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