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原文由 poplar_yang(poplar_yang) 发表:
maybe because your medicine concentration is too low
原文由 heguang(heguang) 发表:原文由 cissie-1986(cissie-1986) 发表:原文由 poplar_yang(poplar_yang) 发表:
PK=4, buffer pH at 4, this medicine is electrical 0;
if this medicine is stable at other pH, you can use buffer pH 10, make it electrical negative, and come out very late after methanol.
I agree with you, but when I tried the borate buffer with pH between 9 to 10, the medicine is also invoved in the methanol. So I feel so depressed.
Be careful, some compounds can dissolve in high pH buffer, but it will encounter the danger of decompose in high pH.
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