主题:【讨论】再次讨论试剂纯度,仁者见仁智者见智!!

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疯子
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我们都知道,常规的化学试剂纯度分为:化学纯,试剂纯,分析纯,优级纯

但我们常见的有色谱纯,HPLC级纯,农残级等等,这些有定义吗?纯度有界限吗?

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有些是有界定的,比如ACS上有规定必须达到什么要求才能算HPLC级别的试剂,还有很多是以应用分的,比如农残级,这个是约定俗成的说法;

还有很多ACS没有规定的试剂,很多就是各家公司的自定义,或者根据某些法规来生产的

还有一些不止是纯度上的要求,比如USP级的化学品
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Grade Definitions

J.T.Baker Grade Definitions  | Macron Grade Definitions 

J.T.Baker Grades 

BAKER ANALYZED™ Reagent—A grade of high quality chemicals for laboratory and specialized industrial use. Every BAKER ANALYZED™ Reagent label provides an Actual Lot Analysis as your guarantee of purity and consistency. Analytical methods used for these determinations are based on standard test procedures described in technical publications. Frequently, improvements developed by J.T.Baker are employed to achieve a higher degree of reliability.

The quality of many BAKER ANALYZED™ Reagents meets or exceeds the requirements set forth by the American Chemical Society (ACS). These products are designated BAKER ANALYZED™ ACS Reagents.

When J.T.Baker product specifications surpass those listed in the ACS, either in tighter purity levels or additional parameters, the statement “exceeds ACS specifications” will be found in the product listings in the catalog, on our Certificates of Analysis, and on our product labels. These tighter limits and additional specifications provide you with improved consistency and characterization of your reagents.

ULTREX®/ULTREX® II—Acids and salts of extremely high purity with low levels of metallic impurities especially for use in ppt trace element analysis. These products are manufactured using state-of-the-art processing and are packaged under clean room conditions. Post-packaging characterization is performed using advanced analytical instrumental methods. A Certificate of Actual Lot Analysis is provided with every bottle.

BAKER INSTRA-ANALYZED™—Acids and standards that are purified and characterized for use in trace element analysis. You receive an Actual Lot Analysis with every BAKER INSTRA-ANALYZED™ Reagent as your guarantee of purity and consistency and as a reference for your analytical blank values.

BAKER ANALYZED™ HPLC Reagent—A group of high purity solvents, buffer salts, and mobile phase modifiers for use in analytical and preparative separations. BAKER ANALYZED™ HPLC Reagents are optimized for all of your Liquid Chromatography applications, including high performance liquid chromatography, gel permeation chromatography and UV-spectrophotometric analysis. These highly characterized reagents are manufactured and tested to assure interference-free separations and have unmatched lot-to-lot consistency. Recalibration or instrument adjustments due to solvent lot changes are minimized. Solvents are controlled for high assay and low UV absorption, fluorescence, residue and water. An Actual Lot Analysis is provided on the label.

BAKER ULTRA RESI-ANALYZED® Reagent—A group of ultra high purity solvents and salts developed for organic residue extraction/concentration procedures. These solvents are fully characterized and lot controlled by ECD, FID, or other method-specific detectors to deliver the highest level of purity and lot-to-lot consistency. Advanced stabilizer systems are incorporated in BAKER ULTRA RESI-ANALYZED solvents to ensure solvent stability and superior performance that is guaranteed through the expiration period stated on the label. BAKER ULTRA RESI-ANALYZED Reagents are suitable for exacting EPA sample cleanup protocols.

BAKER BIO-ANALYZED™ Reagent—A group of high purity solvents and reagents specially purified and carefully tested to assure suitability and reliability in nucleic acid or peptide synthesis. Carefully controlled low water levels and unsurpassed lot-to-lot consistency deliver better synthesis yields and coupling efficiencies.

PHOTREX®—High purity solvents characterized for use in UV, visible and IR spectrophotometry. The label includes the Actual Lot Analysis for UV absorbency at selected wavelengths.

ULTRAPURE BIOREAGENT—A line of high purity reagents tested for use in biotechnology applications, such as electrophoresis, and liquid chromatography. Where applicable, tests include DNase, RNase, Protease, heavy metals and insoluble matter.

BAKER ANALYZED™ Biochemical—High quality biochemicals for research use. An Actual Lot Analysis is provided on the label.

USP, NF and FCC—Chemicals that conform to the requirements of the United States Pharmacopeia (USP), the National Formulary (NF), and the Food Chemicals Codex (FCC). These products are regulated by the Food and Drug Administration. All meet the requirements necessary for their use in pharmaceutical processing and prescription compounding (USP and NF) and for use in food processing operations (FCC).

Multi-Compendial—A grade of product with full compendial testing as appropriate to the US Pharmacopoeia (USP) or National Formulary (NF) and European Pharmacopoeia (PhEur, EP), British Pharmacopoeia (BP) and Japanese Pharmacopoeia (JP) criteria.

Biotech Reagent—A grade of product which is produced under GMP guidelines, but for which there is no USP/NF compendia for the product.

BakerDRY™—A group of anhydrous solvents specifically manufactured and tested for use in organic, organometallic and oligonucleotide sysnthesis. BakerDRY™ solvents meet ACS specifications and are ready to use, eliminating the need for any further purification. These solvents are available in 100 ml and 1 Liter septum-seal bottles and the CYCLE-TAINER® Solvent Delivery System.

Purified—Chemicals of good quality where there are no official standards or compendia, or in some cases, that meet the requirements of former compendia articles.

BAKER—A grade of chemicals for laboratory use, for organic synthesis, and for routine research usually controlled by physical criteria and often by assay (by GC).

Practical—Chemicals of sufficiently high quality for use in diverse applications. Some products may contain intermediates, isomers, or homologs.

Technical—Chemicals of selected commercial quality, useful in some applications.



Macron Grades 

ACS—Reagents meeting the requirements of the American Chemical Society Committee on Analytical Reagents. The specifications listed in this catalog are based on the ACS Reagent Chemicals Ninth Edition. 

AR—The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. If the reagent also meets the requirements of the American Chemical Society Committee on Analytical Reagents, it will be denoted as an AR (ACS) reagent.

AR (ACS) Primary Standard—An analytical reagent of exceptional purity that is specially manufactured for standardizing volumetric solutions and preparing reference standards.

AR Select—A line of acids specifically developed for trace metal analysis; analyzed for up to 32 metals in the low ppb range.

AR Select Plus—The purest grade of Mallinckrodt acids for trace metal analysis. AR Select Plus acids are packaged in glass bottles. These products are tested for 40 elements in the high ppt to low ppb range, ensuring low background interference. 

Certified—A line of stains certified by the Biological Stain Commission. 

ChromAR—Solvents that meet ACS specifications and are suitable for liquid chromatography and UV-spectrophotometry.

CP (Chemically Pure)—Products of purity suitable for use in general applications.

FCC—Products that meet the requirements of the current Food Chemical Codex.

GenAR—A line of reagents specifically developed for use in biotechnology and genetic research.

Lab Grade—A line of solvents suitable for histology methods and general laboratory applications.

Nanograde—Solvents designed for organic residue extraction/concentration procedures that utilize GC/ECD and GC/FID methods.

NF—Chemicals manufactured under current Good Manufacturing Practices (cGMP) and meeting the requirements of the National Formulary.

OR—Organic reagents that are suitable for research applications.

Practical—Products of a purity suitable for use in general applications.

Purified—Chemicals of good quality where there are no current compendia. Most purified chemicals were formerly listed in the USP and NF compendium. The specifications from the former compendia listing have been retained wherever possible.

SilicAR—Silica gel products with physical and chemical characteristics that are optimal for column and thin layer chromatography. 

SpectrAR—A line of solvents designed for UV-Spectophotometric applications.

StandARd—Prepared volumetric and analytical solutions. These solutions are suitable for use in ACS, USP and NF compendial methods and general laboratory applications.

Technical—A grade suitable for general industrial use.

UltimAR—Solvents that meet ACS specifications for liquid chromatography, UV-spectrophotometry, and general purpose use. They are also tested by GC/ECD and GC/FID for use in extraction/concentration sample clean-up protocols for trace-level organic impurities.

USP—Chemicals manufactured under current Good Manufacturing Practices (cGMP) and which meet the requirements of the US Pharmacopeia.

USP/GenAR—A line of chemicals manufactured under cGMP, meet the requirements of the US Pharmacopeia, meet European Pharmacopeia (PhEur, EP) and British Pharmacopeia (BP) specifications where designated, and are endotoxin (LAL) tested where appropriate.

该帖子作者被版主 38666254积分, 2经验,加分理由:谢谢解释
symmacros
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谢谢版主的话题,举个例子,我有瓶优级纯正己烷,纯度大于99%,几乎无什么C5的异构体,但有邻苯(DEHP)。但我另一瓶95%的农残,虽然正己烷的含量不高,但没有邻苯(DEHP)的干扰。
该帖子作者被版主 38666252积分, 2经验,加分理由:鼓励参与
疯子
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原文由 symmacros(jimzhu) 发表:
谢谢版主的话题,举个例子,我有瓶优级纯正己烷,纯度大于99%,几乎无什么C5的异构体,但有邻苯(DEHP)。但我另一瓶95%的农残,虽然正己烷的含量不高,但没有邻苯(DEHP)的干扰。

我买的是色谱纯的,有,但这个是纯度名称的差别吗?估计我也得买农残级了!不过还是不清楚这个纯度是多纯?
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原文由 疯子(3866625) 发表:
原文由 symmacros(jimzhu) 发表:
谢谢版主的话题,举个例子,我有瓶优级纯正己烷,纯度大于99%,几乎无什么C5的异构体,但有邻苯(DEHP)。但我另一瓶95%的农残,虽然正己烷的含量不高,但没有邻苯(DEHP)的干扰。

我买的是色谱纯的,有,但这个是纯度名称的差别吗?估计我也得买农残级了!不过还是不清楚这个纯度是多纯?


不是纯度名称的差别,应用上有区别,另外这两个正己烷有技术参数或COA可以贴出来看下么

HPLC级产品设定为应用满足HPLC紫外检测器,荧光检测器使用,农残级设定为GC-ECD,MS,FID等检测器使用,从色谱系统及检测器上就可以看到两者关注不同,所以使用也不同,这个时候就要看试剂的适用性,比如LC/MS的试剂与GC/MS的试剂要求有些不同

那么农残级试剂的PAEs(邻苯二甲酸酯类)含量低,是有原因的,一方面是MS检测器中邻苯二甲酸酯类会影响实验,所以产品本身就对这个进行控制,还有PEG等;另一方面EPA等一些方法上对邻苯二甲酸酯类有检测,为了满足EPA对检测限的要求,相应配套的试剂也就对这些干扰物有相应的控制。
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杰帝贝柯化工产品
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这个图片上的产品没有讲具体,所以不知道它的纯度是C6烷烃的纯度还是n-C6烷烃的纯度,查了它的COA也没有发现

HPLC的产品很关注的除了纯度,就是紫外的吸收,蒸发残留,当然正己烷由于正相色谱用的比较多,所以水分含量的稳定性也是一个重要方面
睡神
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其实色谱纯不一定纯度特别高,主要是它做实验的时候干扰少
疯子
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原文由 睡神(zhaohu3360083) 发表:
其实色谱纯不一定纯度特别高,主要是它做实验的时候干扰少


纯度应该必会低吧?最起码应该属于优级纯了吧?
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