【Author】 ZHANG Min~1,YUAN Jin-bin~2,HUANG Jia-long~1,HUANG Xing-fa~2,WANG Hui-jun~2(1.Key Laboratory of Modern Preparation of TCM,Ministry of Education,Nanchang 330004,China;2.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China)
【摘要】 目的以吴茱萸碱和吴茱萸次碱为对照品建立吴茱萸生物碱类的高效液相色谱法(HPLC)指纹图谱,为鉴定吴茱萸药材提供依据。方法色谱柱:D iamonsil C18(250 mm×4.6 mm 5μm)。流动相:乙腈-水梯度洗脱,梯度0~5 m in水80%,5~25 m in水80%~35%,25~55 m in水3 5%~0,55~60 m in水0。检测波长225 nm,柱温25℃,进样量10μl。结果测定的20批吴茱萸样品有11个共有峰,共有峰相对保留时间的RSD为1.2%~2.5%,相对峰面积的RSD为2.3%~3.5%。结论该实验首次建立了中药吴茱萸中生物碱成分的HPLC指纹图谱,为不同产地和采收期的吴茱萸药材鉴别提供了科学依据。 更多还原
【Abstract】 Objective Evodiamine and Rutaecarpine were used as reference substances to establish an HPLC fingerprint of alkaloid in Fructus Evodiae for identifying and evaluating its quality.Methods The alkaloid was separated on a Diamosil C18 column（250 mm×4.6 mm,5 μm）.The mobile phase consisted of acetonitrile and distilled water with gradient elution.The flow rate was（1.0） ml/min.The wavelength of UV detection was 225 nm.The column temperature was 25℃ and 10 μl was injected.Results There were ... 更多还原 【关键词】 吴茱萸； 生物碱； 指纹图谱； 【Key words】 Fructus Evodiae； Alkaloid； Fingerprint；
【Author】 ZHAO Yang~1,JIN Feng-yun~1,WU Qing~(2,3*),ZOU Jing~2,LIU Dong-qian~1(1.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China;2.Guizhou Normal University,Guiyang 550001,China;3.Guizhou Provincial Laboratory for Mountainous Environment,Guiyang 550001,China)
【Abstract】 Objective To establish an HPLC method for determination of guanosine and adenosine in Rhizoma Pinellia ternate from different districts in Guizhou.Methods A Diamonsil C18 column（4.6 mm×250 mm,5 μm）was used.The mobile phase was methanol-water（15∶85）,the flow rate was 0.7 ml·min-1,λ=260 nm,and the column temperature was 25℃.Results The linear range of guanosine was within 0.048～0.480 μg,r=0.999 9,the RSD of recovery（n=9） was 2.94%.The linear range of adenosine was within 0.04... 更多还原 【关键词】 高效液相色谱法； 半夏； 鸟苷； 腺苷； 【Key words】 HPLC； Pinellia ternate； Guanosine； Adenosine； 【基金】 贵州省中药现代化资助项目(黔科合农字2005-5034)
【Author】 Lü Ying-nian,WU Ke-feng,DENG Yi-feng,LIANG Nian-ci(Guangdong Medical College,Guangdong Key Lab for Research and Development of Natural Drug,Zhanjiang 524023,China)
【摘要】 目的建立草药半边旗中木犀草素含量的准确测定方法,并测定不同采收期半边旗中木犀草素的含量。方法采用高效液相色谱法,色谱柱为D iamonsil C18,流动相为甲醇水梯度洗脱,流速0.2 m l.m in-1,检测波长为283 nm,柱温度35℃。结果木犀草素进样量在0.062~0.62μg范围内线性关系良好(R2=0.998 7),平均回收率为99.5%(RSD=1.98%)。结论该方法操作简便,结论可靠,可用于半边旗草药的质量控制。 更多还原
【Abstract】 Objective To establish an HPLC method for content determination of luteolin from PSL.Methods The separation was performed on a Diamonsil C18 column,the mobile phase was run with a mixture of methanol（B） and water（A） with a linear gradient elution at a flow rate of 0.2 ml·min-1,the ultraviolet wavelength was 283 nm,and the column temperature was maintained at 35℃.Results The calibration curve showed good linearity in the range of 0.062～0.62 μg（R2=0.998 7）,the aver... 更多还原 【关键词】 高效液相色谱； 半边旗； 黄酮； 木犀草素； 【Key words】 HPLC； Pteris Semipinnata； Flavonoid； Luteolin；
【摘要】 目的建立高效液相色谱(HPLC)法测定克感利咽口服液中栀子苷的含量。方法采用高效液相色谱法,以D ia-monsil(钻石)C18柱为固定相,甲醇-水(29:71)为流动相,流速1 m l/m in,检测波长241 nm,柱温30°C。结果在0.236 4~1.773μg范围内,栀子苷的量与峰面积积分值呈良好的线性关系,r=0.999 9,平均回收率98.96%,RSD=2.31%。结论所建立的方法能准确、快速地进行定量检测,可用于克感利咽口服液的质量控制。 更多还原
【Abstract】 Objective To establish a method for determination of geniposide in Kegan Liyan Oral Solution.MethodsHPLC method was selected to determine the content of geniposide.The chromatographic conditions included Diamonsil C18 column,methanol-water（29:71） as the mobile phase,the detection wavelength at 241nm,flow rate 1.0ml/min and column temperature at 30°C.ResultsThe linear range was 0.236 4～1.773 μg and the average recovery was 98.96%,RSD =2.31%.ConclusionThis method is accurate and quick,a... 更多还原 【关键词】 高效液相色谱法； 克感利咽口服液； 栀子苷； 【Key words】 HPLC； Kegan Liyan Oral Solutio； Geniposide；
【Author】 SONG Ya-li~1,LI Zuo-ping~(2*)(1.Hebei Medical College for Continuing Education,Baoding 071000,China;2.Department of Natural Medicine Chemistry,Hebei Medical University,Shijiazhuang 050017,China)
【Abstract】 Objective To establish a quantitative method for determination of quercitrin in Albizzia julibrissin Durazz.MethodsSample was extracted with methanol,the residue was dissolved with mobile.C18was used as analytic column with methanol-water-acetic acid（45∶55∶1.5） as mobile and detection wavelength was set at 256 nm.ResultsThe peak of quercitrin in sample solution was separated well and linearity in range of 2.54520.36 μg/ml（r=0.999 9）.The average recovery was 98.09%,RSD was 0... 更多还原 【关键词】 合欢； 槲皮苷； 反相高效液相色谱法； 含量测定； 【Key words】 Albizzia julibrissin Durazz； Quercitrin； RP-HPLC； Content determination； 【基金】 河北省自然科学基金资助项目(303470)
【Author】 FU Jun~1,LIANG Ji-zhi~2,LIANG Cong-qing~1 (1.Guangdong College of Pharmacy,Guangzhou 510006,China;2.Guangzhou Hospital of Traditional Chinese Medicine,Guangzhou 510130,China)
【Abstract】 ObjectiveAn RP-HPLC method for the quantitative determination of berberine in Mahuang Tincture was established.MethodsUsing Diamonsil C18column（4.6 mm×250 mm,5 μm）,acetonitrile and a mixed solution of equal amount of 0.01 mol·L-1 sodium heptanesulfonate and 0.02 mol·L-1 potassium dihydrogen phosphate（pH was adjusted to 3.0 by 10% phosphoric acid）（31∶70）as the mobile phase,and the content was detected at a wavelength of 265 nm.ResultsThe linear range of the determ... 更多还原 【关键词】 反相-高效液相色谱法； 马黄酊； 小檗碱； 【Key words】 RP-HPLC； Mahuang Tincture； Berberine；
【Abstract】 ObjectiveInspecting the best soaking time for cutting paeonia.MethodsThe determination of soaked paeonia for different time was carried out on Diamonsil C18column（250 mm×4.6 mm,5 μm）,using acetonitrile-water（17∶83）as mobile phase at a flow rate of 1.0 ml·min-1.The detected wavelength was 230 nm.ResultsPaeoniflorin content of paeonia soaked for 12 hours was higher than the paeonia soaked for 24 hours.ConclusionTo soak 12 hours is available. 更多还原 【关键词】 白芍； 芍药苷； 浸润时间； 【Key words】 Paeonia； Paeoniflorin； Soaking time；
【Abstract】 ObjectiveTo establish a special determination method for folium crataegi extract.MethodsUsing HPLC with Diamonsil C18 column, methanol-water-glacial acetic acid（48∶52∶1） as mobile phase and detecting wavelength at 340 nm.ResultsThe linear range of calibration curve was 13.1879.08μg/ml for the hyperoside（r=0.999 8）.The average recovery was（97.43）% and the RSD was 1.26%（n=5）.ConclusionThe method is simple and accurate,can be used as quantitative analysis for this preparation. 更多还原 【关键词】 金丝桃苷； 山楂叶提取物； 高效液相色谱； 【Key words】 Hyperoside； Folium crataegi extract； HPLC；
【Abstract】 ObjectiveAn HPLC method is developed for the determination of gallic acid in Baixuan Xiatare capsule.MethodsThe separation was performed on Diamonsil-C18 column with a mobile phase of MeOH-0.1%H3PO4（7∶93） and detected at wavelength of 270 nm.ResultsThe linearity range was 0.012 5～0.062 5 μg（r=0.999 1,n=5） and the average recovery of gallic acid was 99.52%,RSD was 0.56%.ConclusionThe method is simple,sensitive and reproducible.It can be used in quality control of ... 更多还原 【关键词】 高效液相色谱法； 百癣夏塔热胶囊； 没食子酸； 【Key words】 HPLC； Baixuan Xiatare Capsule； Gallic acid；
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