OBJECTIVE：To determine the content of baicalin in Ganlu fuzheng oral solution.METHODS：RP-HPLC was adopted.The sample was separated on Platisil ODS（250 mm×4.6 mm,5μm）column with methanol-water-phosphoric acid（45：55：0.2） as mobile phase at a flow rate of 0.8 mL·min-1.The detection wavelength was set at 280 nm and the column temperature was 40℃.RESULTS：The linear range of baicalin was 2.0～14.0μg·mL-1 with an average recovery of 99.44%、99.77%、99.62%（RSD=0.78%,n=3）.CONCLUSION：The method is simple,reliable,stable and reproducible for content determination of Ganlu fuzheng oral solution.
作者： 黄杨 Author： Hang Yang 作者单位： 怀化市药品检验所,湖南,怀化,418000 期 刊： 中医药导报 ISTIC Journal： GUIDING JOURNAL OF TRADITIONAL CHINESE MEDICINE AND PHARMACY 年，卷(期)： 2009, 15(4) 分类号： R927.2 关键词： 高效液相色谱法 骨筋丸(片) 芍药苷 摘要： 目的:建立测定骨筋丸(片)中芍药苷的含量的方法.方法:采用RP-HPLc法,DikmaDiamonsilCl8柱,以乙腈-0.1%磷酸(15:85)为流动相,流速为1.0mL·min-1',柱温:30℃,检测波长:230nm.结果:芍药苷进样量在0.2112-0.7392 靏范围内线性关系良好,r=0.9999,平均加样回收率为98.33%,RSD%为1.51%(n=6).结果:本法专属性强,准确度高,重现性好,可作为该产品质量控制方法.
Objective To determine the contents of the neoeriocitrin, icariin, osthole, naringin, imperatorin in Gushudan granules （ traditional Chinese medicine ）. Methods Diamondsil TM C18 （250 mm × 4.6 mm, 5 μm） column was used with a mobile phase of acetonitrile-water; the flow rate was 1.0 mL·min^- 1 ; the detection wavelength was set at 285 nm. Results The linearity was obtained with good result （r〈0.999 0）. The average recoveries of five active constitutes in Gushudan granules were all in 95.8%- 98.6%, and the RSD were less than 2.1%. Conclusion The validated method has the advantages of simplicity, precision and reliability, allowing quality control of Gushudan granules.
作者： 赵亮[1] LIU En-li[2] 杨翠林[2] LIANG Tai-gang[1] 李青山[2] Author： ZHAO Liang[2] LIU En-li YANG Cui-lin LIANG Tai-gang LI Qing-shan 作者单位： 1.山西医科大学药学院,山西,太原,030001;山东大学齐鲁医院,山东,济南,250012 2.山西医科大学药学院,山西,太原,030001
期 刊： 中华中医药学刊 ISTIC Journal： CHINESE ARCHIVES OF TRADITIONAL CHINESE MEDICINE 年，卷(期)： 2008, 26(8) 分类号： R284 关键词： 海红果 高效液相色谱法 槲皮素 含量测定 摘要： 目的:采用RP-HPLC法测定海红果中黄酮类成分的苷元-槲皮素的含量.方法:采用DiamonsilC18柱(150mm×4.6mm,5μm)作为色谱柱;甲醇:0.05%磷酸(50:50)为流动相;流速为1.20mL/min;柱温为30℃;检测波长为360 nm.结果:该法的线性范围为0.18～1.09μg,r=0.9998;平均加样回收率99.05%,RSD为0.6%;海红果中黄酮苷元的含量为0.0245%,经过提取纯化后含量提高到1.06%.结论:该法用水解后苷元-槲皮素的含量反映海红果中黄酮类成分总量,方法准确,操作简单,结果可靠.
Objective： To establish a method for determination of the content of stemucronatoside K in the roots of Stephanotis mucronata. Methods： The analysis was carried out by RP - HPLC on Diamonsil C - 18 （250mm × 4.6 mm, 5um）, acetonitrile：water （48：52） as mobile phase; wave length was 355 rim; flow rate was 1.0 mL/min. Results： The liner range of stemucronatoside K was 2.024 - 14.168mg. Regression equation was ： Y = 144701X - 9335.5, r = 0. 9998. The average recovery was 98.69% with RSD = 1.48% （n = 9）. Conclusion： The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis for the roots of Stephanotis mucronata.
Objective To establish an RP-HPLC method for the determination of cucurbitacin B laurate in nanoemulsions. Methods The separation was performed with a Diamonsil ODS column （200 mm×4.6 mm, 5 μm） at room temperature, and the mobile phase was consisted of methanol-water （V：V=95：5）, the flow rate was 1.0 mL·min^-1. Cucurbitacin B laurate was detected at 228 nm. Results There was a good linear relationship between A and concentration over the range of 15-150 mg·L^-1 （r=0.999 9, n=5）. The average recovery was 99.98%. The RSD was 0.1%（n=9）. Conclusion This method is applicable for the determination of cucurbitacin B laurate in nanoemulsions.
LIU Yan, LIN Hua-qing, YU Chu-qin, HOU Yue-han（Guangdong Provincial Key Lab of Advanced Drug Deliv- ery, Institute of Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China）
OBJECTIVE： To establish the method for the content determination of main component in Gefitinib dispersible tablet. METHODS： RP-HPLC method was adopted. The separation was achieved by Diamonsil C18 （2） column using acetoni- trile-0.015% trifluoroacetic acid （26：74, pH adjusted to 3.9 with triethanolamine） as mobile phase at the flow rate of 1.0 mL.min-t. The detection wavelength was set at 344 nm and the column temperature was 30 ℃. RESULTS： The linear range of gefitinib was 0.08-6.05 μg.mL-1 （r=0.999 8） with an average recovery of 100.55% （RSD=1.28%, n=9）. CONCLUSION： The method is simple, rapid and reproducible, and it is suitable for the content determination in Gefitinib dispersible tablet.
ZHANG Jun-qing ,CHEN Wen-zhi,JIANG De-xi,ZHANG Dan （1.West China pharmacy school of Si Chuan University,Chengdu 610041,China;2.Affiliated Hospital of Luzhou Medical College,Luzhou 646000,China）
Objective：To establish an RP-HPLC for determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth.Methods： The analytical column was Diamonsil C18 column（250 mm×4.5 mm,5 μm）.The mobile phase consisted of methanol-0.5% ammonium acetate（85∶15）.The detective wavelength was 206 nm.The flow rate was 1.0 mL·min^-1 and the column temperature was 30 degrees.Results： Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.36 μg（r = 0.9995） and 0.65-3.9 μg（r=0.9997）.The average recovery of oleanolic acid was 99.2% with relative standard deviation（RSD） of 8.4% and the ursolic acid was102.5%with RSD of 6.0%（n=9）,the reproducibility test of content was 6.9% and 4.2%（n=6）.Conclusion： This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.
Objective：To develop an HPLC method for determination of naringin in Jinjiawang granules. Method： Dikma DiamonsilTM Cls column was adopted with a mixture of 0. 05 moL·L^-1 potassium dihydrogen phosphate- acetonitrile- strong ammonia （80： 20： 0. 2） as the mobile phase,the flow rate was 1 mL · min^-1 ,and the detection wavelength was 283 nm. Results：The calibration range was 0. 28 - 1.41 μg for naringin （r = 0. 9999 ）. The average recovery was 99.4% ,RSD = 1.4% （n = 6 ）. Conclusion： The method is rapid ,accurate and reliable ,and can be used for the quality control of Jinjiawang granules.
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