ZHANG Ya-zhou, LUO Li-li, DONG Li-sha, CAI Shao-qing（Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;School of Pharmaceutical Sciences, Beijing University, Beijing 100083,China）
OBJECTIVE： To establishes an HPLC method for determination of the contents of Osthol and Berberine in Kangfuyan effervescent suppository. METHODS： HPLC was carried out on a column of Diamonsil （250 mm × 4.6 mm, 5μm） with mobile phase consisted of acetonitrile- 0.2% phosphate acid （52 ： 48,Lauryl sulfonic acid sodium 0.1 g was contained in every 100 mL） with flow rate at 1 mL · min 1.The detection wavelength was set at 322 nm and the column temperature was set at room temperature. RESULTS： The linear range was 0.019 8-0.396 0 μg（r = 0.999 97） for Osthole versus 0.056 65- 1.133 00 μg（ r = 0.999 99） for Berberine. The average recoveries of Osthol and Berberine were 101.06% （RSD = 1.10% ） and 101.88% （RSD = 0.98% ）, respectively. CONCLUSION： This method is simple, accurate and reproducible, and applicable for the quality control of Kangfuyan effervescent suppository.
Objective：To establish RP - HPLC methods for the determination of lamivudinc and its related substances. Method：Separation of lamivudine and its related substances was performed on a Diamonsil C18 column（200 mm × 4. 6 mm,5 μm）. The mobile phase consisted of 0. 025 moL · L^-1 ammonium acetate（pH 3.9 ± 0. 1 ） - methanol（90： 10）. The flow rate was 1.0 mL ·min^- 1 and the detection wavelength was 277 nm. Separation of lamivudine enantiomers was performed on a CYCLOBOND I 2000 RSP column（250 mm × 4. 6 mm,5 μm）. The mobile phase consisted of 0. 05 moL · L^- 1 ammonium acetate （ pH 3.9 ±0. 1 ） - methanol （95 ： 5 ）. The flow rate was 0.8 mL · min^-1 and the detection wavelength was 270 nm. Results：Lamivudine was separated completely from impurities. The linear range of lamivudine was 5 - 50 μg · mL^- 1 （ r = 0. 9997 ）. The average recovery of lamivudine was 100. 1% with RSD of 0. 69% （ n = 9）. The lowest limit of detection was 0. 2 ng · mL^-1. （ - ） - Lamivudine was separated completely from （ ＋ ） -lamivudine. Conclusion：With good sensitivity and selectivity, the method can be applicable for the determination of lamivudine and its related substances.
Objective：To establish an RP - HPLC method for the determination of related substances of tiopronin and to compare the HPLC method with the TLC method in the drug specification. Methods：The chromatographic column was Diamonsil C18 （150 mm×4.6 mm,5 μm）. The mobile phase consisted of 0. 1% phosphoric acid solu- tion- methanol（82： 18） at the flow rate of 1 mL · min-1. The detection wavelength was 210 rim, and the injection volume was 20 μL. Results：Tiopronin was thoroughly separated with its nearest impurity. The calibration curve was linear in the concentration range of 0. 42 - 16. 82 μg · mL -1 （ r = 0. 9999 ） for tiopronin. The detection limit of rio- pronin was 0. 54 ng. Conclusion： Tile RP - HPLC method is superior to the TLC method. The RP - HPLC method is simple, accurate and rapid, and can be used for quality control of tiopronin.
Objective： To develop an RP-HPLC method to determine content of Baicalin in bark of Mufudie [Oroxylum indicum （Linn.） Vent.] . Methods： RP-HPLC method was used. Dikma Diamonsil C18 （250 mm×4.6 mm, 5 μm） was used. The mobile phase consisted of CH3CN- 0.2 % H3PO4 solution （ 26 ： 74） and the flow rate was 1.0 mL ·min^-1.The UV detection wavelength was 280 nm. Column temperature： 30 ℃. Results： The linear range of determination of chrysin was 0.04938-4.938 μg , r = 0.9997 ; the sample solution was stable within 24 h, the precision in a day was good, RSD = 0.87 % ; the average recovery results of chrysin were 99.61%, RSD=2.1% （n=9） . Conclusion： This method was good specialty and precision to determine content of Baicalin in bark of Mufudie, results were accurate and credible.
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