Objective To establish a method for the content determination of butylidenephthalide in Szechwan lovage rhizome. Methods Butylidenephthalide in Szechwan lovage rhizome from different habitats was analyzed with RP-HPLC. A Diamonsil C18 （200 mm× 4.6 mm, 5 μm） column was used to separate the sample by using methanol-water （67： 33） as the mobile phase, The flow rate was 1.0 ml/ min and the detection wavelength 230 nm. Results The calibration curve was linear over the concentration of 6.96 - 69.60 μg/ml for butylidenephthalide （r = 0.9996）. The average recovery of butylidenephthalide was 97.79 % and RSD 1.7%. Cconclusion This method is simple and reproducible, which can be used for the quality control of Szechwan lovage rhizome.
LU Ya-li,ZHAO Jian-bang,SONG Ping-shun （1.Pharmacy College of Lanzhou University,Lanzhou 730000,China;2.Gansu Provincial Institute for Food and Drug Control,Lanzhou 730000,China）
AIM To establish a method for determing the contents of gallic acid and catechin in Rheum palametum,and compare the contents from different habitats including Gansu,Sichuan,Oinghai,Anhui provinces,and Chongqin city.METHODS The samples were extracted with ultrasound method and analyzed on Waters C18 column under gradient elution.The mobile phase was methanol-0.1% ortho-phosphoric acid and the flow rate was 1.0 mL/min.The wavelength of UV detector was set at 280 nm,the column temperature was room temeperature,and the quantification was performed with external standard method.RESULTS The linear range of gallic acid was 0.106-2.125 μg,the average recovery was 99.3%,and the RSD was 0.71%.The linear range of catechin was 0.465-9.296 μg,the average recovery was 98.9%,and the RSD was 2.06%.CONCLUSION The method is quick,simple and accurate,and it can be used for the quality control of Rheum palametum.
PAN Xue-mei1,3,WEI Hui1,LIU Yi2,LIU Su-xiang2,ZHANG Tie-jun2,MA Xu-wei4,HAN Feng-nian4 1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China 3.Department of Pharmacy,Tianjin Hospital,Tianjin 300211,China 4.Shijiazhuang Dongfang Pharmaceuticals Co.,Ltd.,Shijiazhuang 050031,China
LIN Le-wei, JIANG Lin , HAO Da-qing, ZHENG Guo-dong, YANG Xue, WANG Qin, ZHANG Jue-yu （1. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China; 2. School of Life Sciences, Sun Yat-sen University, Gnangzhou 510275, China; 3. Zhongkai College of Agriculture and Engineering, Guangzhou 510225, China ）
AIM：To establish chromatographic fingerprint of Fructus Citri Sarcodactylis from Guangdong province by HPLC and it was expected to fix standard of quality control. METHODS：Analysis was performed on Diamonsil C 18 column （250 mm×4.6 mm,5 μm） with gradient mobile phase of methanol-0.3% acetic acid,fingerprint was finished in 60 min,the monitoring wavelength was at 283 nm and the column temperature was at 25 ℃ with the flow rate of 1.0 mL/min. RESULTS：Establishing the fingerprint of Fructus Citri Sarcodactylis from Guangdong province, 13 common peaks were found in the HPLC fingerprints from different sources. CONCLUSION： This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Fructus Citri Sarcodactylis from Guangdong province.
LIN Le-wei, JIANG Lin, ZHENG Guo-dong（1. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China;2. Guangzhou Medical College, Guangzhou 510182, China）
Objective：To determine the contents of hesperidin, nobiletin and tangeretin in Citrus reticulata ‘Chachi’ from various habitats and different collecting periods（from October to December）and study the dynamic change of three flavonoids constituents. Methods： The HPLC method was used for analysis the contents of flavonoids in Citrus reticulata ‘Chachi’ . The system used a Diamonsil C18 column（250 mm×4.6 mm, 5 μm）with gradient mobile phase of acetonitrile-methanol（80：20）-2% acetic acid. The monitoring wavelength was at 283 nm and 330 nm and the column temperature was at 25 ℃ with the flow rate of 1.0 mL/min. Results： The contents of hesperidin, nobiletin and tangeretin in Citrus reticulata ‘Chachi’ collecting from various habitats descended gradually with the mature of fruit, especially in nobiletin and tangeretin. Conclusion： The method was simple, convenient and can be used to provide some foundation for the quality control of Citrus reticulata ‘Chachi’.
ZHANG Yi-xuan, LI Kai-tong,LI Qiu-yue,SHI Yue （Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100193, China）
Objective：To establish HPLC fingerprint of Folium Pyrrosiae for identification. Methods：The HPLC method was developed with Diamonsil C,s column（250 mm × 4. 6 mm,5 μm）, and a mixture liquid of acetonitrile-0. 8% acetic acid solution as mobile phase in a gradient elution. HPLC fingerprints of 44 samples were analyzed by similarity, cluster and principal component analysis. Results ： The HPLC fingerprint common pattern of Pyrrosia petiolosa, Pyrrosia lingua and common pattern of Pyrrosia sheareri were set up separately. Samples from different species were classified based on the result of cluster and principal component analysis. Fingerprints of Pyrrosia sheareri and Pyrrosia lingua have high degree of similarity, but were different from Pyrrosia petiolosa, while Pyrrosia calvata and Pyrrosia assimlis were classified as adulterants with their dissimilar fingerprints. Conclusion：The method is stable and reliable with a hood reproducibility and provides a reference standard for identifving Folium Pyrrosiae from different habitats and species.
Objective To analyze and identify the chemical components of Trigonella foenum-graeeum L of different producing area by LC-MS. Methods The analysis was performed on an Diamonsil C18 （250mm×4.6mm,5μ）column. The mobile phases were aeetonitrile and HAe and water in gradient elution. ESI source was used and data were acquired in positive and negative mode. Resuits The structures of 11 compounds of Trigonella foenum-graeeum L were identified by direct com- parison with MS of Q-TOF, the standard substances and the data of the literatures. Furthermore, the types of the compounds were flavones,saponins and alkaloids compounds. Conclusion It is a rapid and accurate method that the chemical composi- tions of traditional Chinese medicine can be identified in terms of the separation of high performance liquid chromatography, the accurate molecular weights measured by MS and other information,which can provide a method for active components research for＇TCM and TCM formulae.
YANG Xue-ping, YUAN Hong-ying, LI feng（ 1. Department of Pharmacy, 88 Hospital of PIA, Taian 271000 ; 2. Shandong University of Traditional Chinese Medicine ,Jihan 260014）
Objective ：To study and establish the fingerprints of Lonicera japonica Thunb from different places by HPLC. Methods：The fingerprints of Lonicera japonica Thunb was built by using Diamonsil C18 as analytical column and acetonitrile-0. 05 H3PO4 aqueous in gradient as a mobile phase. The flow rate was 1.0 ml/min. Detecting wavelength was set at 254nm. The temperature of column was at 40 ℃. Results：The mutual mode to HPLC fingerprints was set up, the similarities in 3 batches of Lonicera japonica Thunb samples were all above 0.95. Conclusion ：This methods is simple, stable and reproducible.
我的社区
签到
