主题：【原创】ProElut™ PXA 固相萃取柱检测白酒中赭曲霉毒素A

江南大学用户开发的方法，检测效果棒棒哒，点赞
购买原文有点贵啊$35.95
原文链接：http://www.sciencedirect.com/science/article/pii/S0956713515303157
Quantification of ochratoxin A in Chinese liquors by a new solid-phase extraction clean-up combined with HPLC-FLD method

• Wenyou Zhu^{a, b},
• Cong Ren^a,
• Yao Nie^{a, ,} ,
• Yan Xu^{a, ,}

• ^a State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China
• ^b Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Institute of Biotechnology Industry, College of Life Science & Food Engineering, Yibin University, Yibin, Sichuan 644000, China

Abstract
Ochratoxin A (OTA), one mycotoxin produced by certain filamentous species of Aspergillusand Penicillium, is not known whether existing in Chinese liquors. However, specific method to detect OTA as well as OTA occurrence in Chinese liquors has not been reported so far. For this purpose, based on reversed phase/strong anion-exchange mixed-mode column (ProElut™ PXA), a novel solid-phase extraction (SPE) clean-up method followed by high-performance liquid chromatographic (HPLC) quantification was developed to detect OTA in Chinese liquors. Meanwhile, the matrix effects were evaluated by using the post-extraction spiked method with the signal suppression/enhancement (SSE) as the evaluation index. Under the optimized conditions, no matrix effects were found due to the SSE values below 20%, and the external standard method can be used to determine OTA in Chinese liquors. The recovery rate of spiked samples ranged from 81.6 to 100.8%. Intra-day and inter-day relative standard deviation were below 7.3% and 12.8%, respectively. The limits of detection and quantification were 0.006 and 0.02 μg/L, respectively. This approach was then applied to analyze the 76 samples from different geographical regions in China, including 40 strong aroma-, 30 light aroma- and 6 soy sauce aroma-type raw liquors. The results showed that OTA was detected in 9 samples with a maximum concentration of 0.17 μg/L. Considering that ～12% of the samples were OTA positive, it is necessary to recommend a routine monitoring of this commodity.
Keywords

• Ochratoxin A;
• Distilled liquor;
• Reversed-phase/strong anion-exchange mixed-mode column;
• Matrix effects;
• HPLC-FLD

Chemical compound studied in this article

• Ochratoxin A (PubChem CID: 442530)

Received 7 July 2015, Revised 1 November 2015, Accepted 29 November 2015, Available online 7 December 2015