主题：HPLC/UV

HPLC/UV–Vis/ESI MS for the analysis of textile dyes was successfully applied in a forensic setting. These results demonstrate that LC/MS is a viable method for the analysis of dyes in polyester and acrylic fibers. Parameters were optimized for disperse and basic dye classes that allowed for the separation and identification of dyes through mass spectral and UV–Vis data. Single fibers were successfully extracted and analyzed using these methods, including the use of a novel acetonitrile extraction for disperse dyes from polyester fibers in lieu of pyridine. Finally, a master method was developed that could separate and identify both disperse and basic dye standards. Continuing development of LC/MS analysis for all dye classes as well as the development of an extended spectral database will provide a greater discrimination capability in forensic fiber analysis.
The authors wish to thank Faye Springer (Sacramento County District Attorney's Laboratory of Forensic Services, CA) and S. Kay Obendorf (Cornell University, NY) for their help with manuscript preparation. We wish to thank an anonymous source for donating the acrylic fibers.
This work was sponsored by NSF CHE-0133758, State of California's Local Forensic Laboratory Improvement Program (LFLIP) through Coverdell 02 Federal Funding, and CAC A. Reed and Virginia McLaughlin Endowment Research Award. This work was carried out at Sacramento County District Attorney's Laboratory of Forensic Services.
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