看文献测定毒素用的液质,但是写了一句four serial octadesyl silica columns,4个连续的柱子,这是什么作用?增加保留时间?太夸张了吧?不太懂,请高手指点,一下是原文节选: trichothecenes were quantified by liquid chromatography-mass spectrometry (Model ZQ Quattro Micro, Waters, Milford, MA) with an octadesyl silica column and the mobile phase of acetonitrile/10 mM ammonia acetate water/methanol (5:90:5) for DON or NIV and with four serial octadesyl silica columns and acetonitrile/10 mM ammonia acetate water (25:75) for 3ADON or 15ADON. |