A 200 mg portion of the above freeze-dried aqueous residue was dissolved in 4ml of HPLC grade methanol and chromatographed on a Sephadex LH-20 column (45 cm×2.5 cm I.D.) eluted with methanol.
将200mg上述冻干水性残留溶于4mlHPLC级的甲醇中,经过Sephadex LH-20柱(45 cm×2.5 cm I.D.),用甲醇洗脱。
Methanolic fractions (10 ml each) were collected in test tubes and their absorbance was measured at 280 nm by the use of UV spectrophotometer (UV mini 1240 Shidmazu).
将甲醇洗脱的样品(即样品中溶于甲醇的部分)收集于试管中(每个试管10ml),用紫外分光光度计在280nm处测定吸收度。
Based on the absorbance data, eluates were pooled into major fractions, solvent evaporated and re-dissolved in 1ml of HPLC grade methanol. Samples were prepared as above in duplicate. The samples were filtered through a 0.45_m filter prior to injection (20μl) to the HPLC system.
在吸光度的数据基础上,洗脱物被集中,将溶剂挥发,再用1mlHPLC级甲醇溶解。样品按照上述方法准备。进样前用0.45μm的滤纸过滤,进量为20μl。
Based on the absorbance data这句话让人费解。