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| Column | Cogent Diamond Hydride™, 4µm, 100A | |||||||||||||||||
| Catalog No. | 70000-15P-2 | |||||||||||||||||
| Dimensions | 2.1 x 150 mm | |||||||||||||||||
| Solvents | A: DI water + 0.1% ammonium formate B: 90% acetonitrile/10% DI water/0.1% ammonium formate | |||||||||||||||||
| Gradient |
| |||||||||||||||||
| Post time | 5 min | |||||||||||||||||
| Flow rate | 0.4 mL/min. | |||||||||||||||||
| Sample Peaks | 1. Maleic acid, 115 m/z (M - H)- 2. trans Aconitic acid, 173 m/z (M - H)- 3. cis Aconitic acid, 173 m/z (M - H)- 4. Impurity 173 m/z (M - H)- 5. Fumaric acid, 115 m/z ((M - H)- 6. Citric acid, 191 m/z (M - H)- 7. Oxaloacetic acid, 131 m/z (M - H)- Sample stock solutions were made in acidified DI water at a concentration of 0.2 � 0.3 mg/mL. Samples for analysis were made by diluting the stock 1:100 in 50:50 solution A and B. | |||||||||||||||||
| Detection | ESI � neg - Agilent 6210 MSD TOF mass spectrometer. |
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