紫外可见分光光度计(UV)

主题:【讨论】固体紫外漫反射得到的光谱纵坐标是吸收值 还用Kubelka-Munk转换吗

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wangqun5992
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固体紫外漫反射 得到的原始数据纵坐标是什么?我的仪器测的给的是A(吸收),还能做K-M变换吗?还是直接就可以用测的数据
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aliphd
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可以直接用,因为做之前你已经做了zeor/baseline,0%和100%两个标准已经确定了。
mebutterfly
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原文由 (nktree) 发表:
可以直接用,因为做之前你已经做了zeor/baseline,0%和100%两个标准已经确定了。

测试时选的是FR模式,得到的谱图纵坐标是FR, 可以直接用吗,FR是什么含义,和A有什么关系啊
Spain
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一般FR其实就已经帮你K-M变换了。

The Kubelka-Munk theory is generally used for the analysis of diffuse reflectance spectra obtained from weakly absorbing samples. It provides a correlation between reflectance and concentration. The concentration of an absorbing species can be determined using the Kubelka Munk formula:

F(R) = (1R)²/2R = k/s = Ac/s

R = reflectance
k = absorption coefficient
s = scattering coefficient
c = concentration of the absorbing species
A = absorbance
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2012/3/23 12:19:20 Last edit by spain
juzi618
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原文由 Spain(spain) 发表:
一般FR其实就已经帮你K-M变换了。

The Kubelka-Munk theory is generally used for the analysis of diffuse reflectance spectra obtained from weakly absorbing samples. It provides a correlation between reflectance and concentration. The concentration of an absorbing species can be determined using the Kubelka Munk formula:

F(R) = (1R)²/2R = k/s = Ac/s

R = reflectance
k = absorption coefficient
s = scattering coefficient
c = concentration of the absorbing species
A = absorbance


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p3226105
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原文由 Spain(spain) 发表:
一般FR其实就已经帮你K-M变换了。

The Kubelka-Munk theory is generally used for the analysis of diffuse reflectance spectra obtained from weakly absorbing samples. It provides a correlation between reflectance and concentration. The concentration of an absorbing species can be determined using the Kubelka Munk formula:

F(R) = (1-R)²/2R = k/s = Ac/s

R = reflectance
k = absorption coefficient
s = scattering coefficient
c = concentration of the absorbing species
A = absorbance
你好, 不是很理解,这里的F(R)代表的是什么,是直接测的反射值吗?那这个反射值是与浓度成比例的吗,谢谢
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