主题:【求助】凝胶色谱的问题

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simao118
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凝胶色谱装柱的几个问题,在装柱之前要对凝胶进行溶胀,我准备用G-15的,直接用缓冲液(我用的是tris-hcl)进行沸水溶胀2h,这样可以不呢?还有G-15装一个柱子需要多少克?用缓冲液进行溶胀的时候的用量大概是多少呢?谢谢~~
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小不董
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查到的sephadex G15的信息,不知道是不是和你的一样,这G15没有说明书吗?

Properties
Related Categories
Gel Filtration Chromatography, Gel Filtration Media, Molecular Biology, Protein Chromatography, Proteomics More...
shelf life 
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature 
autoclavable In wet form (pH 7.0) at 120?C for 30 min
packaging 
pack of 100 g
mfr. no. 
GE Healthcare, 17-0020-01
parameter 
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
matrix 
cross-linked dextran
particle size 
40-120 μm (dry)
 
60-180 μm (wet)
working range 
2 - 13
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Description
Analysis Note
www.gelifesciences.com.
Application
Sephadex? is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex? differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex? G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex? G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and Low operating pressures are required.
Features and Benefits
? Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
? Suitable for buffer exchange and cleanup of biological samples, for example peptides, small Proteins, and oligosaccharides larger than 1 500 molecular weight
? Efficient removal of contaminants such as salts, dyes, and radioactive labels
General description
Sephadex? G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules > 1500 molecular weight.
Legal Information
Sephadex is a registered trademark of GE Healthcare
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