主题：【资料】Free-Zone Electrophoresis in Glass Capillaries

  lucas 关于毛细管电泳的开创性的文献，是毛细管电泳技术走向商品化仪器的奠基文献。摘要见下：
A simple theory of zone electrophoresis in open-tubular capillaries is developed. According to this theory, to achieve the highest resolution of zones, tubes with as small an inside diameter as possible should be used in combination with as high an applied voltage as feasible. To testthis approach, we performed electrophoresis In glass capillaries with an internal diameter of 75 m and a length of 100 cm. A special fluorescence detector was used to detect fluorescent zones while they migrated inside the capillary. With the application of 30 kV potentials to this system, rapid and efficient separations of amino acids,peptides, and urinary amines were demonstrated. In all cases fluorescent derivatives were necessary for detection. Preliminary results are encouraging, and with further development of sensitive detection devices, applications in clinical analyses may be feasible.
clasic paper  CZE  LUCAS.PDF