主题：【讨论】HPLC测定头孢噻肟钠

那位仁兄做过注射用头孢噻肟钠，选用的是什么色谱柱，及相应的流动相

    本人采用2010药典方法，使用创新通恒的色谱柱，主峰及杂质峰的拖尾都非常严重。再不改变流动相的情况下，用哪种色谱柱的比较好

  <SPAN style="FONT-SIZE: 10.5pt; COLOR: black; FONT-FAMILY: 'Times New=" New? Roman?;"">  2010药典的色谱条件：<SPAN style="FONT-SIZE: 10.5pt; COLOR: black; FONT-FAMILY: 'Times New=" New? Roman?;""> <SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>用十八烷基硅烷键合硅胶为填充剂；以<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>磷酸盐缓冲液（取<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>无水磷酸氢二钠至<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>量瓶中，加水溶解并稀释至刻度，用磷酸调节<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>值至<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>）－甲醇（<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>：<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;"FONT-SIZE: 10.5pt; FONT-FAMILY: 宋体;" New? Roman?;"New" Roman??>）为流动相；检测波长为<SPAN style="FONT-SIZE: 10.5pt; FONT-FAMILY: 'Times New=" New? Roman?;