主题：【求助】荧光内量子效率为负数

仅仅改变吸收范围，荧光内量子效率从负数变到大于1变到小于1，不知道是哪里有问题。请各位指教。

外量子效率在上述情况下一般在0.2-0.4或者接近0。

附上结果。

Results of Quantum yield calculation
Internal quantum yield: -2.306
External quantum yield: 0.470
Absorptance: -0.204
Amount of absorption: -102.866
Amount of fluorescence: 237.216
Calculation Parameters
Wavelength area (absorption): 530.0 - 720.0 nm
Wavelength area (fluorescence): 550.0 - 700.0 nm
Integrating sphere correction: On
Normalized wavelength: 600 nm
Common with sample and without sample: On
Filter correction: Off
Quantum yield factor: 1.00
File name
Diffuser measurement data:
C:\Program Files\FL Solutions\Correct\diffuser_20101202_164704.FDS
Integrating sphere measurement data (without sample):
C:\Program Files\FL Solutions\Correct\withoutsample_20110510_174143.FDS
Measurement data without sample:
C:\Documents and Settings\Administrator\桌面\withoutsample.FDS
Measurement data with sample:
C:\Documents and Settings\Administrator\桌面\sample2.FDS