原文由 liuqiasnqia(liuqiasnqia) 发表:
克林酶素磷酸酯分析
有没有人做过克林酶素磷酸酯分析呀,买了几根色谱柱都不行,急死了.
是没有保留还是怎么回事?
可以试试如下色谱柱和分析条件:
Column | Cogent Bidentate C8™, 4µm, 100A |
Catalog No. | 40008-15P |
Dimensions | 4.6 x 150mm |
Mobile Phase | 40% Acetonitrile, 60% 10mM Phosphate Buffer, pH 7.5 |
Flow rate | 1.0 mL/minute |
Peaks | Clindamycin |
Injection Volume | 20 µL |
Detection | UV 338 nm |
Temperature | 25°C |
This semi-synthetic antiobiotic is structurally related to lincomycin and the chromatogram shown of clindamycin is from a complex pharmaceutical formulation. Note the excellent peak shape and the selectivity from the excipients. The stability and robustness of the Cogent TYPE-C™ columns makes this an excellent choice for QC applications.