主题：【分享】27.1HPLC法测定黄连降糖片中葛根素的含量

【作者】 夏正燕； 冯瑛； 章曙丹；

【Author】 XIA Zhengyan,FENG Ying,ZHANG Shudan (Zhejiang Research Institute of Chinese Meteria Madica,Hangzhou 310023)

【摘要】 目的测定黄连降糖片中葛根素的含量。方法样品用30%乙醇超声提取进样,色谱柱:DiamonsilC18柱(250mm×4.6mm,5μm),流动相:乙腈-0.5%冰醋酸溶液(梯度洗脱),检测波长:250nm,流速:1mL/min。结果葛根素在0.338～2.366μg之间与峰面积线性关系良好,r=0.999997(n=7),平均回收率100.2%,RSD为1.80%(n=6)。结论该法简便,快速,准确,可用于该制剂及葛根药材的含量测定及质量控制。 更多还原

【Abstract】 Objective To establish a method for the determination of Puerarin in Huanglian Jiangtang Tablets. Methods The sample was extracted with 30 %ethanol. The chromatographic conditions were as follows:Diamonsil C18 chromatographic column(250 mm×4.6 mm,5μm)with a mobile phase of acetonitrile-0.5 %glacial acetic acid,the detection wavelength being at 250 nm and the flow rate being 1.0 mL·min-1. Results A linearity was obtained from 0.338 μg to 2.336 μg of Puerarin in Huanglian Jiangtang Tablets with a ... 更多还原

【关键词】 葛根素； 黄连降糖片； HPLC法； 含量测定；