主题：【分享】27.4HPLC法测定保和口服液中连翘苷的含量

作　　者：傅军 李焕丹 高晓霞 梁从庆 潘志青
FU Jun, LI Huandan, GAO Xiaoxia, LIANG Congqing, PAN Zhiqing  （Guangdong College of Pharmacy,
Guangzhou 510006 ）
摘　　要：目的 建立保和口服液中连翘苷的含量测定方法.方法 采用HPLC法,色谱柱为Diamonsil C18柱,流动相：乙腈-水（29：71）,流速为1.0 mL·min-1,检测波长277 nm,柱温为室温.结果 连翘苷在0.068～0.340μg范围内线性关系良好,相关系数r=0.9996;平均回收率为99.7%（RSD=2.1%）.结论 该方法简便,快速,准确,重复性好,可作为保和口服液的质控指标之一. Objective To establish a method for the determination offorsythin in Baohe Oral Liquid by HPLC. Methods The determination was carried out on a Diamonsil C18 column, with mobile phase of acetonitrile-water（29：71） at room temperature. The flow rate was 1.0mL·min^-1 and the detective wavelength at 277 nm. Results The calibration curves was linear in the range of 0.068-0.340μg （r=0.9996） . The average recovery rate of tested sample was 99.7 % （RSD=2.1%）. Conclusion The method was specific, accurate and precise. It can be used for the quality control of Baohe Oral Liquid. 关 键 词：保和口服液 高效[url=https://insevent.instrument.com.cn/t/5p]液相色谱法[/url] 连翘苷 含量测定 Baohe Oral Liquid Forsythin HPLC Content determination