【Author】 WANG Li(West China School of Pharmacy,Sichuan University,Chengdu 610041,China)WANG Li,XU Ting,TANG Yao(Department of Pharmacy,West China Hospital,Sichuan University,Chengdu 610041,China)ZHANG Shanchuan(West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China)LV Yan(West China School of Public Health,Sichuan University,Chengdu 610041,China)
【Abstract】 OBJECTIVE:To establish an HPLC method for determination of Emodin in Yanyan granules.METHODS:The stationary phase was Diamonsil C18(150mm× 4.6mm,5μ m)column and the mobile phase was methanol-0.1% phosphoric acid(68∶ 32),with detection wavelength set at 254nm.RESULTS:Within the range of 0.26～ 3.90μ g Emodin had a good linearity,and the average recovery was 96.73%(RSD=1.49%).CONCLUSION:The method is accurate,sensitive,reproducible,simple and can be used for the quality control of Yanyan granules. 更多还原 【关键词】 咽炎颗粒； 大黄素； 高效液相色谱法； 含量测定； 【Key words】 Yanyan granules； Emodin； HPLC； Content determination；
【Author】 YIN Guihua(The Affiliated Hospital of Beihua University,Jilin 132001,China) YANG Meilin,ZHONG Chonglin,LIU Shiyue(Jilin Provincial Academy of Traditional Chinese Medicine,Changchun 130021,China)
【Abstract】 OBJECTIVE:To establish a RP-HPLC method for the assay of Jasminoidin in compound frutex abri tablets.METHODS:The separation of Jasminoidin was carried out on Diamonsil C18 under room temperature.The mobile phase consisted of methanol-0.1%H3PO4 solution(22∶78)at a flow rate of 1.0mL·min-1.The detection wavelength was 254 nm.RESULTS:The linear range for Jasminoidin was 0.125～0.623μg(r=0.999 6),and the average recovery was 97.81%(RSD=0.90%,n=9).CONCLUSION:The method is simple,accurate and reproduci... 更多还原 【关键词】 复方鸡骨草片； 栀子苷； 反相高效液相色谱法； 含量测定； 【Key words】 Compound frutex abri tablet； Jasminoidin； RP-HPLC； Assaying；
【Abstract】 OBJECTIVE:To determine plasma concentration of irbesartan by HPLC with fluorescence detection.METHO-DS:Samples were separated on Diamonsil C18 with mobile phase consisted of 0.02mol·L-1 KH2PO4-acetonitrile(50∶50) at a flow rate of 1.0mL·min-1.The fluorescence detector was set at an excitation wavelength of 250nm and an emission waveleng-th of 375nm.The column temperature was 40℃.RESULTS:The linear range for irbesartan was from 1.0 to 1000ng·mL-1 (r=0.999 8).The mean relative recoveries of irbesa... 更多还原 【关键词】 HPLC-荧光检测法； 厄贝沙坦； 血药浓度； 【Key words】 HPLC with fluorescence detection； Irbesartan； Plasma concentration
【Author】 SUN Wenjia,XIA Dongya,GUO Tao(Dept.of Pharmacy,The General Hospital of Shenyang Military Region,Shenyang 110016,China) SUN Wenjia(College of Pharmacy,Dalian Medical University,Dalian 116027,China)
【Abstract】 OBJECTIVE: To establish RP-HPLC method for the determination of fluconazole in human plasma.METHODS: After liquid-liquid extraction,the plasma sample was analyzed on Diamonsil C18 column with column temperature set at room temperature.The mobile phrase consisted of methanol-0.07% aqueous solution of triethylamine(50∶ 50) with a flow rate of 1mL· min-1.The UV detection wavelength was set at 210nm,and phenacetin was used as the internal standard.RESULTS: The linear range of fluconazole was 0.15～ 1... 更多还原 【关键词】 反相高效液相色谱法； 氟康唑； 血药浓度； 【Key words】 RP-HPLC； Fluconazole； Blood concentration；
【Abstract】 OBJECTIVE:To establish a HPLC method for the determination of dolastron mesylate.METHODS:The sample was separated on Diamonsil C18 column.The mobile phase consisted of acetonitrile-water-1mol· L-1 ammonium formate(450∶ 440∶ 110)(pH=8.0 adjusted with trithylamine)with a flow rate of 1.0mL· min-1.The UV detection wavelength was set at 285nm and the sample size was 20μ L.RESULTS:The linear range of dolasetron mesylate was 24～ 56μ g· min-1(r=0.999 6),with average recovery at 99.7%(RSD=0.74%).CONCLUS... 更多还原 【关键词】 高效液相色谱法； 甲磺酸多拉司琼； 含量测定；
【Abstract】 OBJECTIVE:To establish HPLC method for determination of Synephrine in Yanyan buccal tablets.METH- ODS:The stationary phase was Diamonsil C18（150mm×4.6mm,5μm）column and the mobile phase was methanol-water （potassium dihydrogen phosphate 0.6g,sodium dodecylsulphate 1.0g,glacial acetic acid 1mL,add water to 1000mL）=50:50, and we set 225nm as detective wavelength.RESULTS:Within 0.245～2.450μg Synephrine had a good linearity（r=0.9995）,the average recovery was 93.49% with RSD of 1.88%.CONCLU... 更多还原 【关键词】 咽炎口含片； 辛弗林； 高效液相色谱法； 含量测定； 【Key words】 Yanyan buccal tablets； Synephrine； HPLC； Content determination；
【Abstract】 OBJECTIVE: To prepare Total alkaloids of Sophora alopecuroides( TASa) effervescent suppository and to study its quality standard. METHODS: The suppository was prepared with TASa as principal agent, and the contents of the three alkaloids— Oxymatrine, Matrine and Sophocarpine in the suppository were determined by HPLC with DiamonsiL C18( 150mm× 4. 6mm, 5μ m) used as chromatographic column and mobile phase consisted of acetonitrile-0. 05mol· L-1 K3PO4 buffer solution ( 2‰ triethylamine) =10. 5∶ 89... 更多还原 【关键词】 苦豆子总碱； 泡腾栓； 制备； 质量标准； 【Key words】 Total alkaloids of Sophora alopecuroides； Effervescent suppository； Preparation； Quality standard； 【基金】 甘肃省中医药管理局基金项目(2003-GZK-40)
【Abstract】 OBJECTIVE:To establish a RP-HPLC method for the determination of terazosin hydrochloride in human plasma.METHODS:Terazosin hydrochloride and internal standard(parazosin hydrochloride) were extracted from plasma with dichlormethane-diethylether(2:3) for determination.The separation was carried on a Diamonsil C18 column with the mobile phase consisted of 0.05mol· L-1 potassium dihydrogen phosphate buffer solution(pH4.8)-methanol-acetonitrile(61:25:14).The flow rate of the mobile phase was 1.0mL· m... 更多还原 【关键词】 高效液相色谱法； 盐酸特拉唑嗪； 血药浓度； 荧光检测器； 【Key words】 RP-HPLC； Terazosin hydrochloride； Blood concentration of drug； Fluorescence detector；
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