257.9RP-HPLC法测定角花胡颓子中齐墩果酸与熊果酸的含量
作 者:张俊清[1] 陈文芝[2] 蒋德锡[2] 张丹[2]
ZHANG Jun-qing ,CHEN Wen-zhi,JIANG De-xi,ZHANG Dan (1.West China pharmacy school of Si Chuan University,Chengdu 610041,China;2.Affiliated Hospital of Luzhou Medical College,Luzhou 646000,China)
机构地区:[1]海南医学院药学系,海口571101 [2]四川大学华西药学院,成都610041
出 处:《药物分析杂志》 CAS CSCD 2010年第7期 1305-1307页,共3页
Chinese Journal of Pharmaceutical Analysis
基 金:国家支撑计划项目课题(2007BA︱27B06)
摘 要:目的:建立同时测定角花胡颓子中齐墩果酸与熊果酸含量的RP-HPLC法。方法:采用Diamonsil C18(250 mm×4.6mm,5μm),以甲醇-0.5%乙酸铵(85∶15)为流动相,检测波长为206 nm,流速为1.0 mL·min^-1,柱温30℃。结果:齐墩果酸进样量在0.06-0.36μg(r=0.9995)、熊果酸进样量在0.65-3.9μg(r=0.9997)范围内,线性关系良好,齐墩果酸与熊果酸的平均加样回收率分别为99.2%和102.5%,RSD分别为8.4%和6.0%(n=9),重复性试验的含量的RSD分别为6.9%和4.2%(n=6)。结论:该法简便、快速、准确,可用于角花胡颓子的质量控制。
Objective:To establish an RP-HPLC for determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth.Methods: The analytical column was Diamonsil C18 column(250 mm×4.5 mm,5 μm).The mobile phase consisted of methanol-0.5% ammonium acetate(85∶15).The detective wavelength was 206 nm.The flow rate was 1.0 mL·min^-1 and the column temperature was 30 degrees.Results: Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.36 μg(r = 0.9995) and 0.65-3.9 μg(r=0.9997).The average recovery of oleanolic acid was 99.2% with relative standard deviation(RSD) of 8.4% and the ursolic acid was102.5%with RSD of 6.0%(n=9),the reproducibility test of content was 6.9% and 4.2%(n=6).Conclusion: This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.
关 键 词:角花胡颓子 齐墩果酸 熊果酸 HPLC 含量测定
Elaeagnus gonyanthes Benth oleanolic acid ursolic acid HPLC quantitative determination