"The ddPCR technology has great utility as a diagnostic. Unlike most other NAATs, this method requires no external or internal calibration yet delivers a highly accurate estimation of target load. The inclusion of a human target allows a system of internal control that indicates (but does not discriminate between) experimental failures that are due to either technical PCR failure or to the absence of a sample of human origin in the specimen.
We have validated a highly specific quantitative ddPCR assay and applied it to population monitoring of ocular C. trachomatis infections. This method could rapidly be adapted for use in the detection of sexually transmitted C. trachomatis infections, where a higher sensitivity than that which we have estimated would be desirable. This study represents an early example of the usefulness of this next generation digital PCR method in diagnosing infections in clinical specimens within the arena of infectious diseases."
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