主题：[资料]文章:DNA微阵列芯片的应用

APPLICATIONS OF DNAMICROARRAYS IN BIOLOGY
Roland B. Stoughton
Annu. Rev. Biochem. 2005. 74:53–82

Key Words:expression profiling, genomics, genotype, transcription

■ Abstract:DNA microarrays have enabled biology researchers to conduct largescale
quantitative experiments. This capacity has produced qualitative changes in the
breadth of hypotheses that can be explored. In what has become the dominant mode of
use, changes in the transcription rate of nearly all the genes in a genome, taking place in
a particular tissue or cell type, can be measured in disease states, during development,
and in response to intentional experimental perturbations, such as gene disruptions and
drug treatments. The response patterns have helped illuminate mechanisms of disease
and identify disease subphenotypes, predict disease progression, assign function to
previously unannotated genes, group genes into functional pathways, and predict activities
of new compounds. Directed at the genome sequence itself, microarrays have
been used to identify novel genes, binding sites of transcription factors, changes in
DNA copy number, and variations from a baseline sequence, such as in emerging
strains of pathogens or complex mutations in disease-causing human genes. They also
serve as a general demultiplexing tool to sort spatially the sequence-tagged products
of highly parallel reactions performed in solution. A brief review of microarray platform
technology options, and of the process steps involved in complete experiment
workflows, is included.
Fulltext:
Applications of DNA Microarrays in biology.pdf



Figure. Process flow for microarray experiments.