主题：【分享】USP色谱柱类型如何选择？

在工作中常常会遇到眼花缭乱的USP色谱柱类型描述L#，L##，
这些神秘的符号到底代表什么？
不用去论坛求助在线等，我们可以直接去它们的老家查户口，USP官网可以免费查到这些信息，下面我们来看看如何找到他们：
首先进入USP的官网：www.usp.org

现在USP为了方便我大天朝人民浏览，添加了简体中文界面，请在主页左上角选择

看到熟悉的中文，USP好像也亲切一点了，接下来开始寻找免费资源，在导航栏的最右边的“在线商店”里选择“免费资源”

选择色谱柱项目，毫不犹豫的点进去

进行到这里，我们可以看到一堆介绍，可以无视，但是对于第一次访问USP的访客，需要先进行注册才能看到免费的资源，注册的过程很简单，全程中文提示

登录之后，看免责声明，从这开始，没有中文了，不过可以无视，直接Agree就OK了

可以随心选择想要查看的色谱柱了，液相色谱柱都以“L”开头，选择好了之后点SEARCH
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之后可以看到有关该类色谱柱的信息，包括键合相描述，那些品牌的色谱柱属于此分类还有生产该色谱柱的厂商
之后，凭着好人做到底的态度，附上L1到L62的中英文描述对照，免去大家看英文的麻烦：

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USP键合相种类及推荐色谱柱：
L1Octadecylsilane chemically bonded to porous silica or ceramic.
L1十八烷基键合硅烷化学键合于多孔硅胶或陶瓷微粒，3-10u。

L2Octadecylsilane chemically bonded to silica gel of a controlled surfaceporosity that has been bonded to a solid spherical core, 30 to 50 μm indiameter.
L2十八烷基键合硅烷化学键合于表面空隙率一定的键合在紧密球核上的硅胶，粒径30-50u。

L3Porous silica particles, 5 to 10 μm in diameter.
L3多孔硅胶颗粒，粒径5-10u。

L4Silica gel of controlled surface porosity bonded to a solid spherical core,30to 50 μm in diameter.
L4表面空隙率一定的硅胶键合于紧密球核上，粒径30-50u。

L5Alumina of controlled surface porosity bonded to a solid spherical core,30 to50 μm in diameter.
L5表面空隙率一定的氧化铝键合于紧密球核上，粒径30-50u。

L6Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on asolid spherical core, 30 to 50 μm in diameter.
L6强阳离子交换填料：磺化氟代烃聚合物涂渍于紧密球核上，粒径30-50u。

L7Octylsilane chemically bonded to totally porous silica particles, 1.5 to 10 μmin diameter.
L7辛基硅烷化学键合于完全多孔硅胶担体，粒径10u。

L8An essentially monomolecular layer of aminopropyl-silane chemically bonded tototally porous silica gel support, 3 to 10 μm in diameter.
L8氨丙基硅烷单分子层化学键合于完全多孔硅胶担体，粒径10u。

L9Irregular or spherical, totally porous silica gel having a chemically bonded,strongly acidic ation-exchange coating, 3 to 10 μm in diameter.
L910u不规则的完全多孔硅胶具有化学键合的强酸性阳离子交换涂层。

L10Nitrile groups chemically bonded to porous silica particles, 3 to 10 μm indiameter.
L10氰基团化学键合于多孔微粒硅，粒径3-10u。

L11Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm indiameter.
L11苯基化学键合于多孔微粒硅，粒径3-10u。

L12Strong anion-exchange packing made by chemically bonding a quaternary amine toa solid silica spherical core, 30 to 50 μm in diameter
L12由四元胺化学键合于精密硅球核形成的强阴离子交换填料，粒径30-50u。
L13Trimethylsilane chemically bonded to porous silica particles,3 to 10 μm indiameter.
L13三甲基硅烷化学键合于完全多孔硅胶，粒径3-10u。

L14Silica gel having a chemically bonded, strongly basic quaternary ammoniumanion-exchange coating, 5 to 10 μm in diameter.
L14具有强碱性四元胺阴离子交换涂层的10u硅胶化学键合相。

L15Hexyl silane chemically bonded to totally porous silica particles,3 to 10 μm indiameter.
L15己基硅烷化学键合于完全多孔性微粒硅，粒径3-10u。

L16Dimethyl silane chemically bonded to totally porous silica particles, 5 to 10μm in diameter.
L16二甲基硅烷化学键合于完全多孔性微粒硅，粒径3-10u。

L17Strong cation-exchange resin consisting of sulfonated cross-linkedstyrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μm in diameter.
L17由磺化的苯乙烯-二乙烯基苯交连共聚物组成的氢型阳离子交换树脂，粒径7-11u。

L18Amino and cyano groups chemically bonded to porous silica particles,3 to 10 μmin diameter.
L18氨基和氰基化学键合于多孔微粒硅，粒径3-10u。

L19Strong cation-exchange resin consisting of sulfonated cross-linkedstyrene-divinylbenzene copolymer in the calcium form, 9 μm in diameter.
L19由磺化的苯乙烯-二乙烯基苯交连共聚物组成的钙型阳离子交换树脂，粒径7-11u。

L20Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10μm in diameter.
L20二甲氧基丙烷基化学键合于多孔硅胶微粒，粒径5-10u。

L21A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 μm in diameter.
L21刚性球形苯乙烯-二乙烯基苯共聚物，粒径5-10u。

L22Acation exchange resin made of porous polystyrene gel with sulfonic acidgroups, about 10 μm in size.
L22由多孔聚苯乙烯凝胶和磺酸基组成的阳离子交换树脂，粒径约为10u。

L23An anion exchange resin made of porous polymethacrylate or polyacrylate gelwith quaternary ammonium groups, about 10 μm in size.
L23由多孔聚甲基丙烯酸酯或聚丙烯酸酯凝胶及四元胺基组成的阴离子交换树脂，粒径约为10u。

L24A semi-rigid hydrophilic gel consisting of vinyl polymers with numeroushydroxyl groups on the matrix surface, 32 to 63 μm in diameter.
L24由乙烯基聚合物和基质表面的无数羟基组成的半刚性亲水硅胶，粒径32u-63u。

L25Packing having the capacity to separate compounds with a MW range from 100 to5000 daltons (as determined by polyethylene oxide), applied to neutral,anionic, and cationic water-soluble polymers. A polymethacrylate resinbase,crosslinked with poly-hydroxylated ether (surface contained some residualcarboxyl functional groups) was found suitable.
L25能分离MW从100到5000Da化合物的填料（取决于聚环氧乙烷），应用于中性、阴离子和阳离子的水溶性聚合物。聚甲基丙烯酸酯作为树脂基质，与聚羟基化醚（表面含一些残余羧基官能团）进行交联。

L26Butyl silane chemically bonded to totally porous silica particles, 5 to 10 μmin diameter.
L26丁基硅烷键合于全多孔硅胶颗粒上，5～10um粒径

L27Porous silica particles, 30 to 50 μm in diameter.
L27全多孔硅胶颗粒，30～50um粒径

L28A multifunctional support, which consists of a high purity, 100 , sphericalsilica substrate that has been bonded with anionic (amine) functionality inaddition to a conventional reversed phase C8 functionality.
L28混合型填料，氨基基团和C8基团键合于100A孔径的球形硅胶上

L29Gamma alumina, reversed phase, low carbon percentage by weight, alumina-basedpolybutadiene spherical particles, 5 μm diameter with a pore diameter of 80 .
γ-氧化铝，低载碳量反相键合，氧化铝基质的聚丁二烯球形材料，5um粒径，80A孔径

L30Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10 μmin diameter.
L30全多孔硅胶键合乙基硅烷固定相

L31A strong anion-exchange resin-quaternary amine bonded on latex particlesattached to a core of 8.5 μmmacroporous particles having a pore size of 2000 Åand consisting of ethylvinylbenzene cross-linked with 55 % divinyl benzene.
L31季胺基改性孔径2000Å的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂 8.5um粒径，2000A孔径

L32A chiral ligand-exchange packing- L-proline copper complex covalently bonded toirregularly shaped silica particles, 5 to 10 μm in diameter.
L32L-脯氨酸铜配合物共价键合于不规则形硅胶微粒的配位体的交换手性色谱填料，5～10um粒径

L33Packing having the capacity to separate proteins of 4,000 to 400,000 daltons.It is spherical, silica-based and processed to provide pH stability.
L33能够分离分子量4000～40000范围蛋白质分子的球形硅胶固定相, pH稳定性好
L34Strong cation-exchange resin consisting of sulfonated cross-linkedstyrene-divinylbenzene copolymer in the lead form, about 9 μm in diameter.
L34铅型磺化交联苯乙烯－二乙烯基苯共聚物强阳离子交换树脂，9um粒径

L35A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type)molecular monolayer bonded phase having a pore size of 150Å.
L35锆稳定的硅胶微球键合二醇基亲水分子单层固定相，孔径150Å

L363,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5μmaminopropyl silica.
L36 L-苯基氨基乙酸－3,5二硝基苯甲酰键合于5um胺丙基硅胶基质上

L37Polymethacrylate gel packing having the capacity to separate proteins bymolecular size over a range of 2,000 to 40,000D.
L37适合分离分子量2000～40000的聚甲基丙烯酸酯凝胶柱

L38Methacrylate-based size-exclusion packing for water-soluble samples.
L38 分离水溶性样品的甲基丙烯酸酯基质SEC色谱柱

L39Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
L39亲水全多孔聚羟基甲基丙烯酸酯色谱柱

L40Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles,5 μmto 20 μm in diameter.
L40Tris 3,5－二甲基苯基氨基甲酸酯纤维素涂覆多孔硅胶微球，5～20um粒径

L41Immobilized alfa1-acid glycoprotein on sphericalsilica particles, 5 μm in diameter.
L41球形硅胶表面固定α1酸糖蛋白固定相，5um粒径

L42Octylsilane and octadecylsilane groups chemically bonded to porous silicaparticles,5μm in diameter.
L42C8和C18硅烷化学键合于5um多孔硅胶固定相

L43Pentafluorophenyl groups chemically bonded to silica particles, 5 to 10 μm indiameter.
L43 硅胶微球键合五氟苯基固定相，5～10um粒径

L44A multifunctional support, which consists of a high purity, 60 , sphericalsilica substrate that has been bonded with a cationic exchanger, sulfonic acidfunctionality in addition to a conventional reversed phase C8 functionality.
L44混合功能填料,60 Å孔径高纯硅胶基质键合磺酸阳离子交换功能团和C8反相功能团

L45Beta cyclodextrin bonded to porous silica particles, 5 to 10 μm in diameter.
L45β-环糊精键合多孔硅胶微球，5～10um粒径
L46Polystyrene/divinylbenzene substrateagglomerated with quaternary amine functionalized latex beads, about 9 μm to11 μm in diameter.
L46季胺基改性苯乙烯-二乙烯基苯聚合物微球

L47High capacity anion-exchange microporoussubstrate, fully functionalized with atrimethylamine group, 8 μm in diameter.
L47高容量阴离子交换微孔填料，键合三乙基胺集团，8um粒径

L48Sulfonated, cross-linked polystyrene with an outer layer of submicron,porous,anion-exchange microbeads, 15 μm in diameter.
L48磺酸化的交联聚苯乙烯基质并带有键合氨基阴离子交换基团微球的外层的填料，15um粒径

L49A reversed-phase packing made by coating a thin layer of polybutadiene on tospherical porous zirconia particles, 3 to 10 μm in diameter.
L49聚丁二烯涂布于球形多孔氧化锆颗粒制成的反相填料，3～10um粒径

L50Multifunction resin with reversed-phase retention and strong anion-exchangefunctionalities. The resin consists of ethylvinylbenzene, 55 % cross-linkedwith
divinylbenzenecopolymer, 3 to 15 μm in diameter, and a surface area of not less than 350m2/g, substrate is coated with quaternary ammonium functionalized latex particlesconsisting of styrene cross-linked with divinylbenzene.
L50反相，强阴离子混合功能树脂填料，树脂由乙基苯乙烯（55%）与二乙烯基苯共聚物交联构成，3～15um粒径，表面积不小于350 m2/g、孔径2000Å的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂，基质上涂有季胺官能的苯乙烯与二乙烯基苯交联的胶乳颗粒

L51Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silicaparticles,
5to 10 μm in diameter.
L51直链淀粉三-2,5 - 二甲基苯基氨基甲酸酯涂层的多孔球形硅胶颗粒填料，5～10um粒径

L52A strong cation exchange resin made of porous silica with sulfopropyl groups, 5to 10 μm in diameter.
L52键合有磺酸基团的多空硅胶的强阳离子交换填料，5～10um粒径

L53Weak cation-exchange resin consisting of ethylvinylbenzene, 55 % cross-linkedwith
divinylbenzenecopolymer, 3 to 15 μm diameter. Substrate is surface grafted with carboxylicacid and/or phosphoric acid functionalized monomers. Capacity not less than 500μm in diameter.
L53弱阳离子交换树脂，树脂由乙基苯乙烯（55%）与二乙烯基苯共聚物交联构成，3～15um粒径

L54A size exclusion medium made of covalent bonding of dextran to highlycross-linked porous agarose beads, about 13 μm in diameter.
L54葡聚糖共聚的高度交联的多孔琼脂糖珠的体积排阻色谱填料，13um粒径。

L55A strong cation exchange resin made of porous silica coated withpolybutadiene-maleic acid copolymer, about 5 μm in diameter.
L55涂布聚丁二烯马来酸共聚物的多空硅胶基质的强阳离子交换填料，5um粒径

L56Isopropyl silane chemically bonded to totally porous silica particles, 3 to 10μm in diameter.
L56异丙基硅烷化学键合的全多孔硅胶颗粒，3～10um粒径

L57A chiral-recognition protein, ovomucoid, chemically bonded to silica particles,about 5 μm in diameter, with a pore size of 120 angstroms.
L57手性识别的卵类粘蛋白化学键和与硅胶颗粒上，5um粒径，120A孔径

L58Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 7 to 11 μm diameter.
L58钠盐形式的磺化交联的苯乙烯-二乙烯基苯共聚物组成的强阳离子交换树脂，7～11um粒径

L59To separate proteins by molecular weight over the range of 10 to 500 kDa.Spherical 10 μm, silica-based, and processed to provide hydrophiliccharacteristics and pH stability.
L59用于分离大于10～500KDa的蛋白，10um球型硅胶基质，具有亲水性和pH稳定性

L60Spherical, porous silica gel, 3 to 10 μm in diameter, surface has beencovalently modified with palmitamidopropyl groups and endcapped.
L60球形多孔硅叫填料，3～10um粒径，表面用棕榈酰胺丙基（palmitamidopropyl）基团修饰并进行封端处理
L61Hydroxide-selective, strong anion-exchange resin consisting of a highlycross-linked core of 13 μmmicroporous particles, pore size less than 10 , andconsisting of ethylvinylbenzene cross-linked with 55 % divinylbenzene with alatex coatingcomposed of 85 nm diameter microbeads bonded withalkanolquarternary ammonium ions (6 %).
L61羟基选择性，强阴离子交换树脂组成的高度交联的核心为13μm的微多孔性颗粒，孔径小于10，并含有由乙基乙烯基苯与55％的二乙烯基苯交联的键合了季铵离子（6％）的微球。

L62C30 silane bonded phase on a fully porous spherical silica, 3 to 15 μm indiameter
L62C30硅烷键合相的全多孔球形硅胶，3〜15μm粒径