The residues of validamycin in rice leaves is analyzed by high performance liquid chromatography (HPLC) using a UV detector at 210 nm, wavelength, methyl alcohol + Disodium hydrogen phosphate buffer solution ( pH=7, volume ratioV/V= 2:98), flow rate 1.2 mL/min, and methyl alcohol + water (volume ratio is 9:1) use as extraction solvent, purified by C18 solid phase extraction column. The coefficient of variation (CV) is 1.64%-2.98%, average yield is 84.25%-102.25%, and the lower detecting limitation is 0.92 mg/kg.
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