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目的:建立黄芩苷粗品中黄芩苷、黄芩素、汉黄芩苷和汉黄芩素含量的高效
液相色谱(HPLC)测定方法,以便控制其质量。方法:固定相用Agilent ZORBAX SB-C18柱(5μm,4.6mm×250mm),苷流动相为甲醇-水-冰醋酸(49:51:0.2),苷元流动相为甲醇-水-冰醋酸(61:39:0.2),检测波长为275nm。结果:黄芩苷、黄芩素、汉黄芩苷和汉黄芩素线性范围分别是0.102~1.020μg(r=0.9997),0.051~0.510μg(r=0.9994),0.01~0.51μg(r=0.9995),0.02~0.20μg(r=0.9999);平均回收率(n=5)分别为99.12%(RSD为1.55%),101.60%(RSD为2.67%),102.27%(RSD为1.92%/40)和98.51%(RSD为3.13%)。结论:本方法简便、准确,可用于黄芩苷粗品的质量控制。