主题：【求助】吸收池的配对对测量的结果的影响是怎样的?

建议楼主亲自做做试验

比色皿要是有了配对误差要进行校正的。

李昌厚的书上说，“日，英，美等发达国家对石英比色皿的配对误差要求达到0.1T%"

你做标准曲线时，测得的数据都不对，这曲线还有意义吗？

吸收池的验收是必须的，使用过程中也应注意期间核查，否则就容易引入误差了。

据药典说，吸收池必须要配对！
The tolerance on the path length of the cells used is ±0.005 cm. When filled with the same solvent, the cells intended to contain the solution to be examined and the compensation liquid must have the same transmittance.Quantitative absorbance measurements usually are made on solutions of the substance in liquid-holding cells. Since both the solvent and the cell window absorb light, compensation must be made for their contribution to the measured absorbance. Matched cells are available commercially for UV and visible spectrophotometry for which no cell correction is necessary. In IR spectrophotometry, however, corrections for cell differences usually must be made. In such cases, pairs of cells are filled with the selected solvent and the difference in their absorbances at the chosen wavelength is determined. The cell exhibiting the greater absorbance is used for the solution of the test specimen and the measured absorbance is corrected by subtraction of the cell difference.
但另一个问题是，调零不能去除其差异？

我们测过空比色皿的吸光度，一般在小数点第3位上有差别，你可以实际测一下，看是否配套。

原文由 zhouyuhu 发表:
建议楼主亲自做做试验

嗯嗯~~谢谢~~

从仪器的调零和测试原理上分析，在每次使用的参比池和样品池不变情况下：
单光束分光光度仪的吸收池需要配对，不匹配的池子对测试结果有影响；
双光束分光光度仪的吸收池可以不必象单光束仪器一样严格地配对，因为储存基线和测试样品时参比池一般不动，仪器会自动消除吸收池不匹配的影响。

原文由 trfsky 发表:
有人说,吸收池的配对对测量的结果有影响,有些人就说没有影响~~到时是什么回事呢?
还有,吸收池的配对对于吸光系数法与标准曲线法的影响是一样的吗?原理与作用是什么??区别在哪里?

说白了，，就是看下配对比色皿的吸光度是不是一样。一般都有一定误差的。