检测限(CCα)和定量限(CCβ)这两个值按照欧盟2002/657/EC计算的话,在什么情况下用什么方式计算还是一知半解的,不知道大家是做何感想的。
下面是欧盟2002/657/EC中的原话:
The decision limit has to be established according to the requirements for identification or identification plus
quantification as defined under ‘Performance criteria and other requirements for analytical methods’ (part 2).
In the case of substances for which no permitted limit has been established, CCα can be established:
— either by the calibration curve procedure according to ISO 11843 (17) (here referred to as critical value of the
net state variable). In this case blank material shall be used, which is fortified at and above the minimum
required performance level in equidistant steps. Analyse the samples. After identification, plot the signal
against the added concentration. The corresponding concentration at the y-intercept plus 2,33 times the
standard deviation of the within-laboratory reproducibility of the intercept equals the decision limit. This is
applicable to quantitative assays only (α = 1 %),
— or by analysing at least 20 blank materials per matrix to be able to calculate the signal to noise ratio at the
time window in which the analyte is expected. Three times the signal to noise ratio can be used as decision
limit. This is applicable to quantitative and qualitative assays.
In the case of substances an with established permitted limit, CCα can be established:
— either by the calibration curve procedure according to ISO 11843 (17) (here referred to as critical value of the
net state variable). In this case blank material shall be used, which is fortified around the permitted limit in
equidistant steps. Analyse the samples. After identification, plot the signal against the added concentration.
The corresponding concentration at the permitted limit plus 1,64 times the standard deviation of the
within-laboratory reproducibility equals the decision limit (α = 5 %),
— or by analysing at least 20 blank materials per matrix fortified with the analyte(s) at the permitted limit. The
concentration at the permitted limit plus 1,64 times the corresponding standard deviation equal the decision
limit( α = 5 %).
See also Article 5 and point 3.2.
3.1.2.6. Detection capability (CCβ)
The detection capability should be determined according to the requirements for screening, identification or
identification plus quantification as defined (see part 2).
In the case of substances for which no permitted limit has been established, CCβ can be established by:
— the calibration curve procedure according to ISO 11843 (17) (here referred to as minimum detectable value
of the net state variable). In this case representative blank material shall be used, which is fortified at and
below the minimum required performance level in equidistant steps. Analyse the samples. After identification,
plot the signal against the added concentration. The corresponding concentration at the decision limit plus
1,64 times the standard deviation of the within-laboratory reproducibility of the mean measured content at
the decision limit equals the detection capability (β = 5 %),
— analysing at least 20 blank materials per matrix fortified with the analyte(s) at the decision limit. Analyse the
samples and identify the analytes. The value of the decision limit plus 1,64 times the standard deviation of
the within-laboratory reproducibility of the measured content equals the detection capability (β = 5 %),
— where no quantitative results are available, the detection capability can be determined by the investigation of
fortified blank material at and above the decision limit. In this case the concentration level, where only ≤ 5 %
false compliant results remain, equals the detection capability of the method. Therefore, at least 20
investigations for at least one concentration level have to be carried out in order to ensure a reliable basis for
this determination.
In the case of substances for which a permitted limit has been established, CCβ can be established:
— either by the calibration curve procedure according to ISO 11843 (17) (here referred to as minimum
detectable value of the net state variable). In this case representative blank material shall be used, which is
fortified around the permitted limit in equidistant steps. Analyse the samples and identify the analyte(s).
Calculate the standard deviation of the mean measured content at the decision limit. The corresponding
concentration at the value of the decision limit plus 1,64 times the standard deviation of the within-laboratory
reproducibility equals the detection capability (β = 5 %),
— or by analysing at least 20 blank materials per matrix fortified with the analyte(s) at the decision limit. The
value of the decision limit plus 1,64 times the corresponding standard deviation equals the detection
capability (β = 5 %).
See also section 3.2.